奶牛子宫内膜炎致病菌的分离与鉴定及耐药性分析

本试验通过对山东省4个大型奶牛场的60头子宫内膜炎患牛进行调查、采样,并用常规方法对细菌进行分离、培养、鉴定。共分离到细菌59株,其中分离率最高的是埃希氏大肠杆菌10株(16.95%)、葡萄球菌属8株(13.6%)、芽孢杆菌属6株(10.2%)、链球菌属5株(8.5%)(其中溶血性链球菌2株)、棒状杆菌属5株(8.5%)、不动杆菌属5株(8.5%)、双球菌5株(8.5%)、肠杆菌属4株(6.8%)、沙门氏菌3株(5.1%)、克雷伯氏杆菌属2株(3.4%)、耶尔森菌属2株(3.4%)、摩拉杆菌属1株(1.7%),而且分离到了梭菌属3株(严格厌氧菌)。药敏试验分析结果显示,大多数致病菌对喹诺酮类药物、阿莫西林、土霉素及氨苄青霉素等耐药率较高,而对头孢菌素类和氨基糖苷类药物耐药率则较低。

Isolation and Identification of Pathogenic Bacterium and Resistance Analysis of Endometritis in Cows

Sixty cows with endometritis were investigated,through researching the sample of uterus of cows,a total of fifty bacterial isolates were made,and the most frequently bacterial isolated bacteria were Escherichia coli(10),Staphylococcus(8),Bacillus(6),Bacillus(6),streptococcus(5),Klebsiella(2),Corynebacterium(5),Acinetobacter(5),Enterobacter(4),Diplococeus weichselbaum(5),Salmonella(3),Yersinia(2),Morarella(1),and anaerobe: Clostridium(3).The result of drug-sensitive test indicated that majority of pathogen showed higher level resistance to quinolones,amoxicillin,terramycin,ampicillin,but lower-level-resistance to cephalosporins and aminoglycosides.