紫锥菊提取物对奶牛外周血单核细胞增殖的影响

目的]为合理开发利用紫锥菊产品提供理论依据。方法]以早期妊娠的荷斯坦奶牛为试验动物,采用ELISA方法检测不同浓度狭叶紫锥菊提取物（Polinacea^TM）（6.3、20.0、60.0、180.0μg/ml）体外结合刀豆蛋白A（ConA,1μg/ml）和美洲商陆有丝分裂原（PWM,0.6μg/ml）对其外周血单核细胞（PBMC）增殖的影响。结果]不同浓度Polinacea^TM均能促进正常奶牛PBMC增殖,180.0、6.3μg/ml Polinacea^TM刺激PBMC增殖的结果分别较对照高了近10和2倍;不同浓度Polinacea^TM结合ConA均可抑制PBMC增殖,其中180.0μg/ml Polinacea^TM结合ConA对PBMC增殖具有显著抑制作用;20.0、60.0μg/ml Polinacea^TM结合PWM可显著刺激PBMC增殖。结论]紫锥菊提取物对奶牛外周血单核细胞增殖的影响具有剂量依赖性。

Effect of Echinacea Extract on Proliferation of Peripheral Blood Mononuclear Cells of Cows

Objective] The aim was to provide the theoretical basis for the rational development and utilization of Echinacea product.Method]With early gravid holstein cows as the tested animals,the effects of Echinacea extract (PolinaceaTM) with different concn.(6.3,20.0,60.0,180.0 μg/ml) combined with concanavalin A(ConA ,1 μg/ml) and pokeweed mitogen (PWM,0.6 μg/ml) in vitro on proliferation of peripheral blood mononuclear cells(PBMC) of cows were studied with ELISA.Result] PolinaceaTM with different concn.all could promote the proliferation of PBMC of normal cows,the proliferation results of PBMC stimulated by 180.0,6.3 μg/ml PolinaceaTM were higher 10 and 2 times than that of CK resp.PolinaceaTM with different concn.combined with ConA could inhibit the proliferation of PBMC,and 180.0 μg/ml PolinaceaTM combined with ConA had significantly inhibition on proliferation of PBMC.PolinaceaTM with 20.0,60.0 μg/ml combined with PWM could significantly stimulate the proliferation of PBMC.Conclusion] The effect of PolinaceaTM on proliferation of peripheral blood mononuclear cells of cows had dose-dependent manner.