| 锥栗果实双向电泳体系的建立 |
| |
| 引用本文： | 郑诚乐,钟凤林,潘东明,刘小芝.锥栗果实双向电泳体系的建立[J].福建农林大学学报(自然科学版),2010,39(5). |
| |
| 作者姓名： | 郑诚乐 钟凤林 潘东明 刘小芝 |
| |
| 作者单位： | 福建农林大学园艺学院,福建,福州350002 |
| |
| 基金项目： | 国家支撑计划项目(2007BAD07B01); 福建省科技厅资助项目(K02058) |
| |
| 摘 要： | 通过对传统双向电泳技术进行改进和优化,包括对锥栗果实蛋白不同提取方法的优化和不同pH值范围IPG胶条的比较等,以建立适于锥栗果实蛋白质组分析的双向电泳方法.结果表明,使用裂解液直接提取蛋白的方法所获得的双向电泳图谱稳定性和重复性较好,分辨率较高,经银染后可分辨出400多个蛋白点,其等电点主要分布于pH 4-7.
|
| 关 键 词： | 锥栗 蛋白质组 双向电泳 |
Establishment of two-dimensional electrophoresis system for chinquapin fruits |
| |
| ZHENG Cheng-le,ZHONG Feng-ling,PAN Dong-ming,LIU Xiao-zhi.Establishment of two-dimensional electrophoresis system for chinquapin fruits[J].Journal of Fujian Agricultural and Forestry University,2010,39(5). |
| |
| Authors: | ZHENG Cheng-le ZHONG Feng-ling PAN Dong-ming LIU Xiao-zhi |
| |
| Institution: | ZHENG Cheng-le,ZHONG Feng-ling,PAN Dong-ming,LIU Xiao-zhi(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
| |
| Abstract: | The conventional two-dimensional electrophoresis technology was improved in order to develop an appropriate approach for proteomics of chinquapin fruits.The study was done by optimizing different protein extraction methods and comparing IPG strips at different pH values.With the improved protein extraction method,a stable,reproducible and high resolution 2-D electrophoresis map was obtained.After silver staining,more than 400 protein spots were detected,with their isoelectric points(pI) mainly at pH 4-7. |
| |
| Keywords: | chinquapin(Castanea henryi Rehd & Wils ) proteome two-dimensional electrophoresis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
