| 铁皮石斛组培育苗与组培苗培植技术研究 |
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| 引用本文： | 罗万业,罗万周,魏锦秋,陈桂琼,朱昔娇.铁皮石斛组培育苗与组培苗培植技术研究[J].广东林业科技,2014,30(2):78-81. |
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| 作者姓名： | 罗万业 罗万周 魏锦秋 陈桂琼 朱昔娇 |
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| 作者单位： | 广东省梅州市林业科学研究所,广东省梅州市大埔县高陂镇林业站,广东省梅州市林业科学研究所,广东省梅州市林业科学研究所,广东省梅州市林业科学研究所 |
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| 摘 要： | 对铁皮石斛组织培养育苗和组培苗培植技术进行研究,结果表明:在MS培养基中添加6-BA 1.0mg/L+NAA 0.1 mg/L+IAA 0.5 mg/L+50 g/L椰子汁有利于芽苗的增殖;在1/2MS培养基中添加6-BA0.1 mg/L+IBA 1.0 mg/L+NAA 0.2 mg/L+50 g/L椰子汁有利于小苗生根和植株生长。搭建大棚移植铁皮石斛组培苗,采用高架苗床,选用松树皮和花生壳(v/v=4∶1)为基质,移栽成活率可达95%以上。
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| 关 键 词： | 铁皮石斛 组织培养 培植技术 |
| 收稿时间： | 3/2/2014 12:00:00 AM |
| 修稿时间： | 2014/3/24 0:00:00 |
Study on the Techniques of Tissue Culture and Cultivation of Dendrobium officinale |
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| Luo Wanye,Luo Wanzhou,Wei Jinqiu,Chen Guiqiong and zhuxijiao.Study on the Techniques of Tissue Culture and Cultivation of Dendrobium officinale[J].Forestry Science and Technology of Guangdong Province,2014,30(2):78-81. |
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| Authors: | Luo Wanye Luo Wanzhou Wei Jinqiu Chen Guiqiong and zhuxijiao |
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| Institution: | Guangdong Province Meizhou City Forestry Science Institute,Guangdong Meizhou,Guangdong Province Meizhou City Forestry Science Institute,Guangdong Meizhou, |
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| Abstract: | The tissue culture and cultivation techniques of Dendrobium officinale were studied, the results showed that: the MS medium supplemented with 6-BA 1.0 mg/L NAA 0.1 mg/L IAA 0.5 mg/L 50 g/L coconut juice was good for bud proliferation; the 1/2MS medium supplemented with 6-BA 0.1 mg/ L IBA 1.0 mg/L NAA 0.2 mg/L 50 g/L coconut juice was good for rooting and growth of seedlings.The transplantion survival rate could reach above 95%,when the plantlets of D. officinale were planted under the conditions of greenhouse, elevated seedbed and growing media with pine bark peanut shell (v/v =4:1). |
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| Keywords: | Dendrobium officinale tissue culture cultivation techniques |
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