| 茄链格孢菌侵染马铃薯叶片过程的细胞学观察 |
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| 引用本文： | 左豫虎,史洁,韩青梅,康振生,台莲梅,刘铜. 茄链格孢菌侵染马铃薯叶片过程的细胞学观察[J]. 中国蔬菜, 2011, 1(14): 35-40 |
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| 作者姓名： | 左豫虎 史洁 韩青梅 康振生 台莲梅 刘铜 |
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| 作者单位： | 1.黑龙江八一农垦大学，黑龙江大庆 163319|2西北农林科技大学植物保护学院，陕西省农业分子生物学重点实验室，陕西杨凌 712100 |
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| 基金项目： | 黑龙江省农垦总局科技攻关项目(HNK10A-03-07) |
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| 摘 要： | 采用光学显微镜和电镜技术系统研究了茄链格孢菌对马铃薯叶片的侵染过程及超微结构特征。结果表明：接种2 h后，分生孢子开始萌发，分生孢子各个位置都可萌发产生芽管|接种6 h后，菌丝顶端出现附着孢|接种8 h后，菌丝从细胞间凹陷处直接侵入感病品种东农303叶片表皮细胞内|接种24 h后，受侵染寄主细胞中的菌丝向相邻细胞扩展蔓延，感病品种细胞内含物及各类细胞器基本完全消解。在抗病品种克新1号上，茄链格孢菌的侵染情况与感病品种基本一致，但发生时间明显较感病品种推迟，寄主细胞内的菌丝数量明显少于感病品种|并且在接种24 h后出现细胞壁加厚的防卫反应，在感病品种上没有观察到防卫反应现象。说明抗病品种对茄链格孢菌具有一定的抗侵入和抗扩展能力。
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| 关 键 词： | 马铃薯 茄链格孢菌 侵染 超微结构 |
Cytology Observation on Process of Potato Leaves Infected by Alternaria solani |
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| ZUO Yu-hu , SHI Jie , HAN Qing-mei , KANG Zhen-sheng , TAI Lian-mei , LIU Tong. Cytology Observation on Process of Potato Leaves Infected by Alternaria solani[J]. China Vegetables, 2011, 1(14): 35-40 |
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| Authors: | ZUO Yu-hu SHI Jie HAN Qing-mei KANG Zhen-sheng TAI Lian-mei LIU Tong |
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| Affiliation: | ZUO Yu-hu1,SHI Jie1,HAN Qing-mei2,KANG Zhen-sheng2,TAI Lian-mei1,LIU Tong1(1Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China,2Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Plant Protection,Northwest Agriculture & Forestry University,Yangling 712100,Shaanxi,China) |
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| Abstract: | The infection process and ultra-structure of Alternaria solani on potato(Solanum tuberosum L.)leaves were observed by microscope,scanning and transmission electron microscope.The results showed that conidia began to germinate,and germ tube start to form 2 h after inoculation.Appressorium formation in the hypha tip was exhibited 6 h after inoculation.The germ tubes directly penetrated the host epidermal cells junctions and formed infection hypha on leaf of susceptive variety'Dongnong 303'8 h after inoculatio... |
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| Keywords: | Potato Alternaria solani Infection Ultra-structure |
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