| PrP106-126对BV-2小胶质细胞NO含量影响及作用机制 |
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| 引用本文： | 李玉荣,武现军,吴浩,陈立功,霍书英,高玉红.PrP106-126对BV-2小胶质细胞NO含量影响及作用机制[J].东北农业大学学报,2013,44(3):114-118. |
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| 作者姓名： | 李玉荣 武现军 吴浩 陈立功 霍书英 高玉红 |
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| 作者单位： | 1. 河北农业大学动物医学院 农业部动物疫病病原生物学华北科学观测实验站,河北保定,071001
2. 保定市畜牧水产局,河北保定,071000 |
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| 摘 要： | 朊蛋白多肽PrP106-126可激活小胶质细胞并产生活性物质。探讨PrP106-126作用于BV-2小胶质细胞对NO生成状况影响,从酶学角度检测其作用机制。在细胞培养液中加入50μmol.L-1PrP106-126,培养48 h后检测培养液NO含量,采用实时定量RT-PCR检测细胞内iNOs和nNOs在mRNA表达水平。结果表明,PrP106-126显著提高细胞培养液中NO含量(9.34倍,P<0.01),且iNOs和nNOs表达水平均极显著提高(11.60倍和4.36倍,P<0.01),从而为解释朊病发生机制提供基础数据。
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| 关 键 词： | 朊病毒 PrP106-126(Prionpe ptide106-126) 小胶质细胞BV-2 NO iNOs nNOs |
Effect of PrP 106-126 on contents of NO in BV-2 microglia |
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| LI Yurong
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Wu Xianjun
,
Wu Hao
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Chen Ligong
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Huo Shuying
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Gao Yuhong.Effect of PrP 106-126 on contents of NO in BV-2 microglia[J].Journal of Northeast Agricultural University,2013,44(3):114-118. |
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| Authors: | LI Yurong Wu Xianjun Wu Hao Chen Ligong Huo Shuying Gao Yuhong |
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| Institution: | 11.School of Veterinary Medicine,Hebei Agricultural University;North China Research Center of Animal Epidemic Pathogen Biology,China Agriculture Ministry.Baoding Heibei 071001,China;2.Animal Husbandry and Fishery Bureau of Baoding,Baoding Hebei 071000,China) |
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| Abstract: | PrP106-126 can increace cell viability of of microglia and the activated microglia can release cytokines,but the metabolic alterations of microglia remain poorly documented.A synthetic peptide corresponding to amino acid 106-126 of prion protein(PrP) has been shown to be toxic to neurons.In this study,VB-2 cell were used,the effects of PrP106-126 on contents of NO in BV-2 cells were investigated in vitro.The experiment was divided into three groups(Control,SCR,PrP106-126).The content of NO was significantly(P<0.01) increased when grown in media with PrP 106-126 at 48 h.The expression of iNOs and nNOs was examined at mRNA levels at 48 h.The results showed that exposure of PrP106-126 enhanced the level of NO(934%,P<0.01),the expression of iNOs(1160%,P<0.01)and nNOs(436%,P<0.01),and this may partially account for the pathogenesis of prion disease. |
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| Keywords: | prion protein Prion peptide 106-126 (PrP106-126) BV-2 microglia NO iNOs nNOs |
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