| M13噬菌体肽库扩增及兔抗血清制备 |
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| 引用本文： | 任晓峰,马晓微.M13噬菌体肽库扩增及兔抗血清制备[J].东北农业大学学报,2013,44(3):79-82. |
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| 作者姓名： | 任晓峰 马晓微 |
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| 作者单位： | 东北农业大学动物医学学院,黑龙江哈尔滨,150030 |
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| 基金项目： | 国教育部新世纪优秀人才项目，教育部科学技术研究重点项目 |
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| 摘 要： | 为制备M13噬菌体多克隆抗体,将噬菌体十二肽原始文库进行大量扩增,作为免疫原制备兔抗M13的多克隆抗体血清并进行间接ELISA鉴定。结果表明,该多抗效价达1 1 048 576,说明此多抗可与扩增噬菌体文库发生很好的抗原抗体反应。将制备的噬菌体M13多克隆抗体与商业化M13抗体水平比较,制备多抗与商业化M13抗体效果相当。本研究成功制备兔抗M13噬菌体多克隆抗体,为深入研究噬菌体展示技术提供了材料。
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| 关 键 词： | 噬菌体肽库 兔抗M13噬菌体血清 间接ELISA |
Amplification and polyclonal antibody preparation of M13 phage display library |
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| REN Xiaofeng
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MA Xiaowei.Amplification and polyclonal antibody preparation of M13 phage display library[J].Journal of Northeast Agricultural University,2013,44(3):79-82. |
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| Authors: | REN Xiaofeng MA Xiaowei |
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| Institution: | (School of Veterinary Medicines, Northeast Agricultural University, Harbin 150030, China) |
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| Abstract: | In order to generate polyclonal antibodies of the M13 Phage,one M13 of Ph..D.-12TM Phage Display Peptide Library was amplified and used to immunize a rabbit to generate polyclonal antibody.Indirect ELISA analysis showed that the titer of the polyclonal antibody was approximately 1: 1 048 576,showing that the anti-M13 antibody had a high titer.Compared this polyclonal antibody with a Rabbit polyclonal antibody(Rb pAb) to M13 Bacteriophage Coat Proteins(commercialization),the binding activity of the produced polyclonal antibody to the identical target was as good as that of the Rb pAb to M13 Bacteriophage Coat Proteins.In the study,polyclonal antibody to this M13 of phage library was successfully generated and such phage polyclonal antibody is important material for functional analysis with phage. |
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| Keywords: | phage peptide library polyclonal antibodies of the M13 indirect ELISA |
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