| 鸭肝炎病毒单克隆抗体的研制 |
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| 引用本文： | 杨萍萍,宋敏训,艾武,马秀丽,崔言顺.鸭肝炎病毒单克隆抗体的研制[J].中国预防兽医学报,2006,28(2):212-216. |
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| 作者姓名： | 杨萍萍 宋敏训 艾武 马秀丽 崔言顺 |
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| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018
2. 山东省农科院,家禽研究所,山东,济南,250023 |
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| 摘 要： | 以反复冻融、氯仿处理、滤膜过滤、PEG反透析浓缩和分子筛层析的方法纯化DHV免疫BALB／c小鼠，同时作为包被抗原建立了筛选抗DHV阳性杂交瘤细胞株的间接ELISA方法，经特异性和重复性试验，效果良好。经一系列融合、筛选，成功获得阳性杂交瘤细胞株4株，并制备出了腹水，分别命名为285、2E8、5E6和6C11。特性鉴定结果表明4株单抗ELISA效价均比较高且特异性好，中和特性稍差，和两株Ⅰ型标准毒以及在山东分离到的几株病毒均有交叉反应。
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| 关 键 词： | 鸭肝炎病毒 单克隆抗体 间接ELISA |
| 文章编号： | 1008-0589（2006）02-0212-05 |
| 收稿时间： | 2004-10-25 |
| 修稿时间： | 2004年10月25 |
Preparation and identification of monoclonal antibodies against duck hepatitis virus |
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| YANG Ping-ping,SONG Min-xun,AI Wu,MA Xiu-li,CUI Yan-shun.Preparation and identification of monoclonal antibodies against duck hepatitis virus[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(2):212-216. |
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| Authors: | YANG Ping-ping SONG Min-xun AI Wu MA Xiu-li CUI Yan-shun |
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| Abstract: | The BALB/c mic.e were immunized with DHV-YZ which purified by freezing and melting repeatedly, chloroform distilling, membrane filtrating, inverse dialyzing and chromatography with Sephadex G-200.Meanwhile, coated with the same purified antigen, I-ELISA for detecting positive hybridoma cells was established. 4 hybridoma cell lines were obtained after fusion, detection and 3 sub-cloning. They were named , 2B5, 2E8, 5E6 and 6C11, respectively Character tests showed that the ELISA titers and specialities of 4 monoclone antibodies are very good, but virus neutralization characters are feeble. In addition, they all have cross interaction with two standard DHV-I strains and several strains isolated from Shandong province. |
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| Keywords: | duck hepatitis virus monoclonal antibody indirect-ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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