我国南方烟区烟草青枯病菌产细菌素特性的研究

CHARACTERISTICS OF BACTERIOCIN PRODUCED BY PSEUDOMONAS SOLANACEARUM ISOLATES COLLECTED FROM THE MAIN TOBACCO-GROWING REGIONS IN SOUTH CHINA

Twenty five isolates of Pseudomonas solanacearum from the main tobacco-growing re-gions in southern China were assayed for the production of bacteriocin.Results showedthat all isolates tested were able to produce bacteriocin and to have sensitivity tobacteriocin.Among them strains 3-5 and 4-3 were the best in the production ofbacteriocin.Their antagonistic range was 95.8%.They might be used for the biocontrol ofP.solanacearum.The sensitivity of strains 4-11 and 4-13 to bacteriocin was higher and thesensitive ranges were 83.3% and 75%,respectively.The two strains should be good indica-tors for the bacteriocin production of other strains.On the basis of inhibition of isolates2-3,3-5,4-11 and 4-13,the isolates tested were grouped into 7 bactriocin types.The mainone was bacteriocin type 4,next comes bacteriocin type 1.There was obvious difference inthe sensitivity to bacteriocin among isolates from different varieties and regions,but no dif-ference in the production of bacteriocin.There was no difference in the production ofbacteriocin,but existed significant sensitivity to bacteriocin among isolates of differentbiotypes and reaction types.The antagonism between isolates from the same regions or vari-eties was stronger than that from different regions or varieties.