一个水稻生物产量突变体的遗传分析

目的]对1个水稻生物产量突变体进行遗传分析。方法]对转Bar基因水稻后代的筛选和鉴定的过程中,在T1代群体10个株系中发现1个生物产量突变株系;采用除草剂Basta喷洒及PCR扩增等方法对该株系进行遗传分析并进行农艺性状考察。结果]该基因突变除了使株型增高外,径秆也较粗,开花较早,分蘖多,穗大,生物产量高。该株系分离比率为3∶1,符合孟德尔遗传分离规律。PCR分子检测证实,突变性状与T-DNA插入的目的基因(Bar)没有共分离,表明突变体不是由目的基因的T-DNA插入所引起的。结论]该研究有助于克隆该突变体诱发基因及理解其对生物产量影响机制。

Study on the Ganetic Characteristics of a Biomass Mutant in Oryza sativa

Objective] The study aimed to reveal the genetic model of a biomass mutant in Oryza sativa.Method] In the process of screening and identification of Bar-transgenic rice,a biomass mutant was found in 10 lines of T1 progenies.The mutant was investigated for genetic analysis and agronomic traits by herbicide spraying and PCR amplification.Result] The segregation ratio is consistent with mendelian law(3∶1).The mutant assumes not only higher plant height,wider straw and earlier florescence,but also more tillers,bigger spikes and resultantly higher biomass.PCR detections indicate that no co-segregation was observed between mutant traits and target gene(Bar) of T-DNA inserted,proving that the mutant is not caused by the insertion of T-DNA containing target gene(Bar).Conclusion] Our study may avail to understand the cloning of mutant gene and the mechanism of the mutant gene on biomass.