水稻螟虫对4种Bt杀虫蛋白的敏感性测定

采用人工饲料喂毒法和浸叶喂毒法测定了二化螟、大螟和稻纵卷叶螟初孵幼虫对4种Bt杀虫蛋白的敏感性。二化螟对Cry1Ab、Cry1Ac、Cry1C和Cry2A的LC50分别为1.739、2.6452、.794 ng/mg和15.965 ng/mg;大螟依次为2.455、10.046、11.803 ng/mg和35.359 ng/mg;稻纵卷叶螟依次为4.000、1.611、69.031μg/mL和12.708μg/mL。作者就不同水稻螟虫对不同Cry蛋白的敏感性差异及其在转基因抗虫作物中的应用作了讨论。

Susceptibility of rice lepidopterans to four insecticidal proteins from Bacillus thuringiensis

The susceptibility of the neonates of rice lepidopterans,striped stem borer Chilo suppressalis(Walker),pink stem borer Sesamia inferens(Walker) and leaffolder,Cnaphalocrocis medinalis(Guenée) to four Cry proteins,i.e.Cry1Ab,Cry1Ac,Cry1C,and Cry2A,from Bacillus thuringiensis were bioassayed using diet-feeding method and leaf-immersion method.The LC50 values of Cry1Ab,Cry1Ac,Cry1C,and Cry2A to C.suppressalis were 1.739 ng/mg,2.645 ng/mg,2.794 ng/mg,and 15.965 ng/mg,respectively;and those to S.inferens were 2.455 ng/mg,10.046 ng/mg,11.803 ng/mg,and 35.359 ng/mg,respectively;and to C.medinalis,4.000μg/ml,1.611μg/ml,69.031μg/ml,and 12.708μg/ml,respectively.Finally,the difference in susceptibility of rice lepidopterans to Cry proteins and its application to transgenic insect-resistant crops were also discussed.