嗜酸乳杆菌的体外抑菌研究

目的]为研究嗜酸乳杆菌的抑菌机理提供一个好的途径。方法]以大肠杆菌和金黄色葡萄球菌为指示菌株,用MRS培养基培养分离纯化嗜酸乳杆菌,利用单层琼脂平板扩散法检测嗜酸乳杆菌在不同pH值条件下对大肠杆菌、金黄色葡萄球菌的抑菌作用。结果]在37℃的恒温箱中培养24 h后,通过测量抑菌圈大小,可以得知嗜酸乳杆菌对大肠杆菌、金黄色葡萄球菌有明显的抑菌作用。在适宜的酸性条件下,嗜酸乳杆菌对大肠杆菌的抑制作用明显,且随着酸度的增强其抑菌效果愈加明显。嗜酸乳杆菌对金黄色葡萄球菌有明显的抑制作用,但pH值的变化对其抑菌效果影响不大。结论]在适宜的生长条件下,嗜酸乳杆菌对一些致病菌具有抑制作用。

Research on the In Vitro Bacteriostasis of Lactobacillus acidophilus

Objective] The purpose was to supply a good approach for studying the bacteriostatic mechanism of Lactobacillus acidophilus.Method] With Escherichia coli and Staphylococus aureus as indicative strains,MRS media were used to culture,isolate and purify L.acidophilus.The monolayer agar plate diffusion method was used to detect the bacteriostasis of L.acidophilus on E.coli and S.aureus under different pH values.Result] After culturing in calorstat at 37 ℃ for 24 h,it could be known through measuring the sizes of bacteriostatic circles that L.acidophilus had obvious bacteriostasis on E.coli and S.aureus.Under the suitable acidic condition,the bacteriostatic effect of L.acidophilus on E.coli was obvious and its bacteriostatic effect was in evidence increase along with the increment of acidity.L.acidophilus had obvious bacteriostasis on S.aureus,but the change of pH value had little influence on its bacteriostatic effect.Conclusion] Under the suitable growth condition,L.acidophilus had bacteriostasis on some pathogenic bacteria.