| 猪O型口蹄疫基因工程疫苗候选株的构建及其免疫原性分析 |
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| 引用本文： | 李平花,白兴文,孙普,李冬,卢曾军,包慧芳,曹轶梅,付元芳,陈应理,谢宝霞,殷宏,刘在新.猪O型口蹄疫基因工程疫苗候选株的构建及其免疫原性分析[J].畜牧兽医学报,2011,42(11). |
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| 作者姓名： | 李平花 白兴文 孙普 李冬 卢曾军 包慧芳 曹轶梅 付元芳 陈应理 谢宝霞 殷宏 刘在新 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点实验室,国家口蹄疫参考实验室,兰州730046 |
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| 基金项目： | 农业行业专项，国家"863计划" |
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| 摘 要： | 近年来,O型口蹄疫病毒已演化出多种谱系.目前应用的疫苗已不能有效保护多谱系口蹄疫的流行,这给我国猪口蹄疫的防控带来了极大的困难.为了进一步发展免疫原性好、抗原谱广的猪O型口蹄疫疫苗候选株,本研究以O/HN/93现用疫苗毒株的感染性克隆为骨架,构建了含VP3(58位)和VP1(43、48、137、139、140、141和142位)氨基酸改造的全长克隆.线性化的全长cDNA和T7RNA聚合酶质粒共转染BHK细胞后获得拯救病毒.RT-PCR和乳鼠致病性试验表明拯救病毒遗传稳定,具有与亲本病毒相似的致病性.用拯救的基因工程病毒灭活疫苗免疫猪28 d后分别用中国谱系、泛亚谱系和缅甸98谱系猪源毒的流行毒攻击,结果均获得了完全保护(16/16).O/HN/93灭活疫苗免疫猪能完全保护泛亚谱系和缅甸98谱系猪源病毒的攻击(16/16),但不能完全保护(12/16)中国谱系猪源病毒的攻击.结果表明基因工程病毒制备的灭活疫苗提高了对中国型猪源谱系病毒的免疫保护,拓展了抗原谱,是具有良好开发前景的疫苗候选株.
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| 关 键 词： | 猪口蹄疫 基因工程病毒 构建 免疫原性分析 |
Construction of Genetically Engineered Vaccine Candidate of Type O Foot-and-Mouth-Disease Virus of Pig and Analysis of Its Immunogenicity |
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| LI Ping-hua,BAI Xing-wen,SUN Pu,LI Dong,LU Zeng-jun,BAO Hui-fang,CAO Yi-mei,FU Yuan-fang,CHEN Ying-li,XIE Bao-xia,YIN Hong,LIU Zai-xin.Construction of Genetically Engineered Vaccine Candidate of Type O Foot-and-Mouth-Disease Virus of Pig and Analysis of Its Immunogenicity[J].Acta Veterinaria et Zootechnica Sinica,2011,42(11). |
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| Authors: | LI Ping-hua BAI Xing-wen SUN Pu LI Dong LU Zeng-jun BAO Hui-fang CAO Yi-mei FU Yuan-fang CHEN Ying-li XIE Bao-xia YIN Hong LIU Zai-xin |
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| Institution: | LI Ping-hua,BAI Xing-wen,SUN Pu,LI Dong,LU Zeng-jun,BAO Hui-fang,CAO Yi-mei,FU Yuan-fang,CHEN Ying-li,XIE Bao-xia,YIN Hong,LIU Zai-xin (State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agriculture,National Foot-and-Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agriculture Sciences,Lanzhou 730046,China) |
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| Abstract: | The outbreaks of type O foot-and-mouth disease(FMD) severely affect development of Chinese hog industry,its causative agent is type O foot-and-mouth disease virus(FMDV),which has been evolved several lineages.The available FMD vaccines in China cannot afford sufficient protection against viruses of multi-lineages,which exert important obstacle to disease control.In order to develop vaccine candidate with characteristics of good immunogenicity and broad spectrotype,we constructed a full-length cDNA clone wit... |
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| Keywords: | Foot-and-mouth disease genetically engineered virus construction immunogenicity analysis |
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