里氏木霉与黑曲霉混合发酵产纤维素酶的研究

研究了利用里氏木霉和黑曲霉混合培养的形式产纤维素酶,以两个菌种的不同接种比和延迟黑曲霉的接种时间来寻找两个菌种发挥最大协同作用的结合点.以农林废弃物之一的玉米秸秆为底物,经过蒸汽爆破预处理后,用作产酶碳源.以里氏木霉单一培养与黑曲霉单一培养为参照进行对比研究.结果表明,黑曲霉接种较里氏木霉延迟48h,里氏木霉与黑曲霉接种量比为5: 1时,滤纸酶活最高,达3.295IU/mL,高于里氏木霉单一培养(2.480IU/mL),β - 葡萄糖苷酶活达1.010IU/mL,也远远高于里氏木霉单一培养(0.243IU/mL).本实验充分证明里氏木霉与黑曲霉混合培养产酶是可行的,并优于单一菌种培养.

Cellulase Production from Mixed Fermentation of Trichoderma reesei RUT C-30 and Apsergillus niger NL02

Cellulase production from mixed fermentation of Trichoderma reesei and Aspergillus niger was investigated. The balance point of the two strains was established by delaying inoculation time of A.niger and using different inoculum ratios. The time courses of cellulase production by monoculture of T.reesei and A.niger were used as controls. It was found that the highest filter paper activity(FPA), 3.295 IU/mL, was obtained when delay in A.niger inoculation was 48h and volumetric inoculum ratio of T.reesei versus A.niger was 5: 1, higher than the FPA of the monoculture of T.reesei, 2.480IU/mL. The β-glucosidase activity(β-GA) also increased from 0.243IU/mL of the monoculture of T.reesei to 1.010IU/mL of the mixed culture aforementioned. It was demonstrated that the mixed fermentation of T.reesei and A.niger was feasible and superior to the monoculture of T.reesei or A.niger.