| 银杏愈伤组织诱导与生长的优化试验 |
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| 引用本文： | 胡燕梅,夏景波,韩晓红,周全.银杏愈伤组织诱导与生长的优化试验[J].中国农学通报,2010,26(24):47-51. |
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| 作者姓名： | 胡燕梅 夏景波 韩晓红 周全 |
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| 作者单位： | 武汉生物工程学院生物工程系,武汉430415 |
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| 基金项目： | 武汉市高校科学研究资助项目，湖北省教育厅科学技术研究项目 |
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| 摘 要： | 采用正交试验研究了四种外植体、基本培养基、萘乙酸（NAA）、6-苄基嘌呤（6-BA）4因素4水平及银杏幼叶汁和胚乳汁对银杏愈伤组织诱导及生长的影响。结果表明：银杏愈伤组织诱导和生长的最佳组合均是以子叶为最佳外植体，而培养基最佳组合分别是MS+ NAA 3.0 mg／L + BA 2.0 mg／L和SH+ NAA 3.0 mg／L + BA 1.5 mg／L；四种因子影响次序是外植体 ＞基本培养基＞NAA ＞ 6-BA；银杏胚乳汁促进愈伤组织生长，而银杏叶汁却抑制愈伤组织生长。
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| 关 键 词： | 台湾 台湾 食品安全 监管 质量认证 |
| 收稿时间： | 2010/4/22 0:00:00 |
| 修稿时间： | 6/5/2010 12:00:00 AM |
Optimization of the Ginkgo Callus Induction and Growth |
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| Hu Yanmei,Xia Jingbo,Han Xiaohong,Zhou Quan.Optimization of the Ginkgo Callus Induction and Growth[J].Chinese Agricultural Science Bulletin,2010,26(24):47-51. |
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| Authors: | Hu Yanmei Xia Jingbo Han Xiaohong Zhou Quan |
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| Institution: | ( Department of Bioengineering , Wuhan Bioengineering Institute , Wuhan 430415) |
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| Abstract: | Ginkgo biloba L. callus induction and growth were studied by orthogonal test of 4 kinds of explants, minimal medium, NAA （NAA）, 6-benzyladenine （6-BA） four factors and four levels, and two other factors of ginkgo leaf juice and endosperm juice in order to obtain good callus clones. The experiment results showed that： the orthogonal test showed the effect sequence of four kinds of factors was explants minimal medium NAA 6-BA for callus induction and growth, of which with the cotyledons as explants in the medium SH ＋ NAA 3.0 mg/L＋BA 1.5 mg/L the callus growth state was the best of the induction rate of 100% and biomass 345.5 g/L; ginkgo endosperm juice could improve the growth and the biomass reached 545.5 g/L, but leaf juice inhibitd the growth of callus. |
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| Keywords: | Ginkgo biloba orthogonal test callus ginkgo leaf juice ginkgo endosperm juice |
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