| 应用碱性抽提法和羟基磷灰石柱层析法提纯质粒DNA |
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| 引用本文： | 裴青生,邰连桂.应用碱性抽提法和羟基磷灰石柱层析法提纯质粒DNA[J].青海畜牧兽医杂志,1991,21(5):8-10. |
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| 作者姓名： | 裴青生 邰连桂 |
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| 作者单位： | 青海省畜牧兽医科学院，青海省畜牧兽医科学院，青海省畜牧兽医科学院 |
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| 摘 要： | 用碱性抽提法和羟基磷灰石柱层析法,加以改进,总结出一套简便易行、低花费的质粒提纯方法。此法适用于一般条件试验室,可进行质粒的批量制备,所提纯质粒经限制性内切酶消解和转化试验,其产量(900μg/500 ml)、纯度和生物学活性均可满足重组DNA的需要。
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| 关 键 词： | 碱性抽提 质粒DNA 羟基磷灰石柱 |
USING IMPROVED ALKALINE LYSIS AND HYDROXYAPTITE CHROMATOGRAPHY TO PURIFY PLASMID DNA |
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| Pei Qingsheng et al..USING IMPROVED ALKALINE LYSIS AND HYDROXYAPTITE CHROMATOGRAPHY TO PURIFY PLASMID DNA[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,1991,21(5):8-10. |
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| Authors: | Pei Qingsheng |
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| Institution: | Qinghai Academy of Animal Science and Veterinary Medicine |
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| Abstract: | We developed a simple, inexpensive and improved purifyine plasmid method. It is suitable for general laboratories and can be used to get large amount of plasmid. The purified plasmid can be digested and transformed by restriction endoucleases. The production is 900 ug/500ml. The purity and biological activity are available for recombinant DNA. |
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| Keywords: | Alkaline lysis HA chromatography Plasmid DNA |
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