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| 酶动力学方法在黄曲条跳甲抗性监测中的应用 | |
| 引用本文： | 郑丽祯,傅建炜,李建宇,林泽燕,占志雄.酶动力学方法在黄曲条跳甲抗性监测中的应用[J].中国农学通报,2010,26(8):226-229. |
| 作者姓名： | 郑丽祯 傅建炜 李建宇 林泽燕 占志雄 |
| 作者单位： | 1. 福建省农业科学院植物保护研究所,福州,350013;福建农林大学应用生态研究所,福州,350002 2. 福建省农业科学院植物保护研究所,福州,350013 3. 福建农林大学应用生态研究所,福州,350002 |
| 摘 要： | 摘要：利用酶标仪动力学方法测定4个菜区黄曲条跳甲的总酯酶、乙酰胆碱酯酶活性分布频率，并与浸叶法测定的药剂敏感性进行比较分析，结果表明利用酶标动力学方法能够快速测定黄曲条跳甲总酯酶、乙酰胆碱酯酶活性在不同活性区段的频率分布，通过分析总酯酶活性大于3.2⊿D(450nm)?min-1?insect-1、乙酰胆碱酯酶活性小于0.016⊿D(405nm)?min-1?insect-1的个体数可以快速监测黄曲条跳甲田间种群的药剂敏感性水平高低。 |
| 关 键 词： | 鸡致病性大肠杆菌 鸡致病性大肠杆菌 O血清型 药敏试验 |
| 收稿时间： | 2009-10-13 |
| 修稿时间： | 2009-11-18 |
Application of enzymatic kinetics method in resistance monitoring of striped flea beetle, phyllotreta striolata (Coleoptera: Chrysomelidae) |
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| Zheng Lizhen,Fu Jianwei,Li Jianyu,Lin Zeyan,Zhan Zhixiong.Application of enzymatic kinetics method in resistance monitoring of striped flea beetle, phyllotreta striolata (Coleoptera: Chrysomelidae)[J].Chinese Agricultural Science Bulletin,2010,26(8):226-229. | |
| Authors: | Zheng Lizhen Fu Jianwei Li Jianyu Lin Zeyan Zhan Zhixiong |
| Institution: | 1Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013; 2Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002 |
| Abstract: | Frequency distributions of general esterase and acetylcholinesterase in striped flea beetle,phyllotreta striolata,from 4 regions were detected with enzymatic kinetics method in a microplate reader, and were compared with the insecticide susceptibility tested by leaf dipping methods. The results indicated that the former method was a rapid detection of insecticide susceptibility of striped flea beetle by analyzing individual number on the general esterase with an activity of more than 3.2⊿D（450nm）（min·insect）, while acetylcholinesterase less than 0.016⊿D（405nm）（min·insect）. |
| Keywords: | resistance monitoringzz |
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