Regeneration and multiplication of Albizia procera Benth. through organogenesis |
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Authors: | SL Swamy JL Ganguli S Puri |
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Institution: | (1) Department of Forestry, I.G. Agricultural University, Raipur (C.G.), 492 006, India;(2) Department of Entomology, I. G. Agricultural University, Raipur (C.G.), 492 006, India |
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Abstract: | Vegetative propagation techniques are recognized as indispensable tools for mass multiplication of important multipurpose
trees adopted in different agroforestry systems. Albizia procera, one among important species, is difficult to propagate commercially either by stem / root cuttings or layering. A study
was undertaken to develop procedure for its in vitro regeneration through organogenesis. Explants collected from 15±2 yr-old mature plus trees and from 15 days old juvenile seedlings
were regenerated with exogenous application of different hormones. Epicotyl and hypocotyl explants excised from juvenile seedlings
showed higher callusing than axillary bud and shoot tip explants derived from mature trees. Benzylaminopurine (BA) at 3 μg/l
was most effective, which induced hundred percent callusing in epicotyl and hypocotyl explants in 1/2 Murashige and Skoog
(MS) medium. Callus originated from axillary buds and apical shoot tips of mature trees failed to form organs, however callus
derived from epicotyl and hypocotyl explants proliferated and formed de novo shoots and leaflets. A concentration of 3 μg/l of BA was found effective for shoot proliferation. Shoots grew vigorously
in 2 μg/l gibberellic acid (GA3) treatment and rooted in 1/2 MS medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Rooting
was most successful on medium supplemented with 6 μg/l IBA alone on which 93.3% of the shoots formed roots. Sand or vermiculite
supplemented with 4 ml of yoshida solution proved as best hardening media, which recorded 70-80% survival of plantlets. One
year old tissue culture raised plants had comparatively more height, collar diameter, biomass, and root shoot ratio than plants
raised from cuttings and seeds of the same age. The procedures enumerated provide a basis for the development of in vitro techniques for rapid multiplication of A. procera.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Afforestation Callus Morphogenesis Rhizogenesis Tissue culture Vegetative propagation |
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