| Abstract: | New methods for the microdetermination of ascorbic acid are described. The 3 proposed methods based on the fact that N-chlorosuccinimide in acid, alkaline or neutral medium readily oxidizes ascorbic acid. The mechanism of the reaction is discussed. The proposed methods are simple rapid, and sufficiently accurate to determine concentrations as low as 10 micrograms of ascorbic acid. The experimental error does not exceed +/- 2%. The methods were also used for the determination of ascorbic acid in such biological fluids as bloody, urine or edible fruits with an error not exceeding +/- 5%. |
