| 鸡传染性支气管炎病毒HN99株血清学和免疫学特性的研究 |
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| 引用本文： | 高文明,崔保安,李新生,魏战勇,徐端红.鸡传染性支气管炎病毒HN99株血清学和免疫学特性的研究[J].中国畜牧兽医,2007,34(9):81-83. |
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| 作者姓名： | 高文明 崔保安 李新生 魏战勇 徐端红 |
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| 作者单位： | 河南农业大学牧医工程学院，郑州 450002 |
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| 摘 要： | 在鸡胚肾细胞上对HN99株病毒进行了克隆纯化，经检测无外源病原污染。应用血清交叉中和试验确定了IBV HN99株的血清型，并用免疫保护试验进行了验证，结果证明HN99株与T株属同一血清型，与TJ、SD、湖北等地方分离株有一定的相关性。不同免疫剂量的效力试验结果表明用0.2 ml剂量的HN99灭活苗接种试验鸡，能够对HN99攻毒提供100%的保护，证明HN99株具有良好的免疫原性。
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| 关 键 词： | 鸡传染性支气管炎病毒 HN99株 血清型 免疫原性 |
| 文章编号： | 1671-7236（2007）09-0081-03 |
| 修稿时间： | 2007-03-16 |
The Immunological Properties and Serotyping of Infectious Bronchitis Virus HN99 Strain |
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| GAO Wen-ming,CUI Bao-an,LI Xin-sheng,WEI Zhan-yong,XU Duan-hong.The Immunological Properties and Serotyping of Infectious Bronchitis Virus HN99 Strain[J].China Animal Husbandry & Veterinary Medicine,2007,34(9):81-83. |
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| Authors: | GAO Wen-ming CUI Bao-an LI Xin-sheng WEI Zhan-yong XU Duan-hong |
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| Institution: | College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | IBV HN99 strain was multiplied and purified by chick embryo kidney cell(CEKC),and the SPAFAS Jinan laboratory certified it was pure.Virus neutralization test(VN) revealed that the serotype of HN99 strain was similar to IBV T strain.The inactivated vaccine of HN99 strain was prepared and it showed absolutely safety,moreover,chickens were immunized with the vaccine at different dose by muscle injection,three weeks later,100 %protection were induced after challenging with HN99 strain,the results indicated that the IBV HN99 strain had fine Immunological Properties. |
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| Keywords: | IBV HN99 strain serotype immunological properties |
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