金属离子对霍山石斛类原球茎增殖及植株再生的影响

 采用单因素浓度试验和正交试验研究了Mg²⁺ 、Ca²⁺ 、Fe²⁺ 、Mn²⁺和Zn²⁺离子对液体培养霍山石斛类原球茎增殖的影响。结果表明, 5种金属离子对类原球茎增殖影响的主次顺序为Fe²⁺ >Mn²⁺ >Mg²⁺ >Ca²⁺ > Zn^{2 +} , 促进类原球茎增殖的优化组合为Mg²⁺ 1.5 mmol·L ^-1、Ca²⁺ 4.5 mmol·L ^-1、Fe²⁺ 0.1 mmol·L ^-1、Mn²⁺ 0.5 mmol·L ^-1、Zn²⁺ 0.06 mmol·L ^-1。经优化的液体培养基增殖的类原球茎能保持植株再生能力, 平均每克类原球茎增殖后可再生植株1 985.2株, 是相同时间内对照类原球茎再生植株数的4.2倍。

Effects of Metal Ions on Protocorm-like Body Proliferation in Liquid Culture of Dendrobium huoshanense and Plantlet Regeneration

This research is concerned with optimization of Mg²⁺ , Ca²⁺, Fe²⁺, Mn²⁺ and Zn²⁺ for protocorm-like body proliferation in liquid culture of Dendrobium huoshanense by one factor-atatime and orthogo nalmatrixmethods. Results showed that, except for Zn²⁺, the effects of othermetal ions on PLB proliferation were significant and the order was Fe²⁺ >Mn²⁺ >Mg²⁺ > Ca²⁺ > Zn²⁺ . The optimal concentration combination for PLB proliferation was determined as 1.5 mmol·L ^-1 Mg²⁺ , 4.5 mmol·L ^-1 Ca²⁺ , 0.1 mmol·L ^-1Fe²⁺, 0.5 mmol·L ^-1 Mn²⁺ and 0.06 mmol·L ^-1 Zn²⁺. Proliferated PLBs remained the potential of plant regeneration. The amount of regenerated plantlets from pergram PLBs through proliferation in optimized liquid medium was 1 985.2 and was about 4.2 times that from PLBs that proliferated on origina lmedium.