| 野龙竹茎中黄酮类化合物的组织化学定位及其提取工艺研究 |
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| 引用本文： | 王明悦,施蕊,杨宇明,王娟,闫修瑜,吴义军. 野龙竹茎中黄酮类化合物的组织化学定位及其提取工艺研究[J]. 林业调查规划, 2013, 38(1): 14-16,21 |
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| 作者姓名： | 王明悦 施蕊 杨宇明 王娟 闫修瑜 吴义军 |
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| 作者单位： | 1. 西南林业大学,国家林业局西南生物多样性保育重点实验室,云南昆明650224
2. 云南省林业科学院,云南昆明,650201 |
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| 基金项目： | 国家林业局948项目(2008-4-30)“竹子重要性状的基因工程研究技术引进”;云南省科技计划项目2009CD073;云南省中青年学术技术带头人后备人才培养项目(2010CI016)共同资助 |
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| 摘 要： | 以野龙竹竹茎为材料，采用组织化学定位方法，研究野龙竹茎中黄酮类物质的积累分布及含量；采用超声波萃取竹茎中的总黄酮，比较各因素对总黄酮提取率的影响，影响顺序为乙醇体积分数〉萃取温度〉料液比〉超声萃取时间。通过正交试验确定最佳萃取条件为：乙醇体积分数为70％，萃取温度为50％，超声萃取时间为15min，料液比为1：25，总黄酮的平均含量为10．36mg／g。
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| 关 键 词： | 组织化学定位 黄酮类化合物 超声波 正交设计 野龙竹 |
Histochemistry Positioning and Extraction Process of Flavonoid Compounds in the Stem of Dendrocalanus Semiscandens |
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| WANG Ming-yue , SHI Rui , YANG Yu-ming , WANG Juan , YAN Xiu-yu , WU Yi-jun. Histochemistry Positioning and Extraction Process of Flavonoid Compounds in the Stem of Dendrocalanus Semiscandens[J]. Forest Inventory and Planning, 2013, 38(1): 14-16,21 |
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| Authors: | WANG Ming-yue SHI Rui YANG Yu-ming WANG Juan YAN Xiu-yu WU Yi-jun |
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| Affiliation: | 1(1.SFA Key Lab of Biodiversity Conservation in Southwest Region of China,Southwest Forestry University, Kunming 650224,China;2.Yunnan Academy of Forestry,Kunming 650201,China) |
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| Abstract: | Taking Dendrocalanus Semiscandens as material, histochemistry positioning method was used to test the accumulation of flavonoids compounds distribution condition in the stem of Dendrocalanus Semi- scandens, the total flavonoids was extracted by using ultrasonic, and the impact of various factors on the extraction rate also were analyzed, results showed that the effect order was followed as alcohol concentra- tion 〉 extraction temperature 〉 ratio of solid to solvent 〉 ultrasonic time. The optimum condition which was 70% of ethanol concentration, 50℃ of extraction temperature, 15 min of ultrasonic extraction time, 1 ： 25 of the solid-liquid ratio, 10.36mg/g of the average content of total flavonoids was confirmed. |
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| Keywords: | Histochemical positioning flavonoids compounds ultrasonic orthogonal design Dendro- calanus Semiscandens |
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