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感染萧氏松茎象的金龟子绿僵菌菌株的初步筛选
引用本文:童应华,陈顺立,林强.感染萧氏松茎象的金龟子绿僵菌菌株的初步筛选[J].林业科学,2010,46(1).
作者姓名:童应华  陈顺立  林强
作者单位:1. 福建农林大学林学院,福州,350002
2. 福建省森林病虫害防治检疫总站,福州,350003
基金项目:福建省自然科学基金项目(Z0516020);;福建省科技厅重点项目(2006N0002);;福建省科技厅重点项目(2004N013)
摘    要:A total of 12 strains of Metarhizium anisopliae Sorokin were tested for pathogenicity to Hylobitelus xiaoi,and a superior strain was used to infect trials in a forest.The results indicated that the strain Ma1291-2 had strong pathogenicity to H.xiaoi after treatment for 30 d by dipping inoculation with (1.5±0.5)×108 conidia·mL-1.The correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were respectively 70.59%,70.81% and (1.90±0.34)×108 conidia individual,and the quickes...

关 键 词:萧氏松茎象  金龟子绿僵菌  致病力  菌株筛选  

Preliminary Screen of Virulent Strains of Metarhizium anisopliae against Hylobitelus xiaoi
Tong Yinghua,Chen Shunli,Lin Qiang.Preliminary Screen of Virulent Strains of Metarhizium anisopliae against Hylobitelus xiaoi[J].Scientia Silvae Sinicae,2010,46(1).
Authors:Tong Yinghua  Chen Shunli  Lin Qiang
Institution:1.College of Forestry/a>;Fujian Agriculture and Forestry University Fuzhou 350002/a>;2.General Station of Forest Disease and Pest Control and Quarantine of Fujian Province Fuzhou 350003
Abstract:A total of 12 strains of Metarhizium anisopliae Sorokin were tested for pathogenicity to Hylobitelus xiaoi,and a superior strain was used to infect trials in a forest.The results indicated that the strain Ma1291-2 had strong pathogenicity to H.xiaoi after treatment for 30 d by dipping inoculation with (1.5 ±0.5)×10~8 onidia·mL~(-1).The correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were respectively 70.59%,70.81% and (1.90±0.34)×10~8 conidia individual,and the quickest median lethal times (LT_(50)) were 13.89 d.The median lethal concentration (LC_(50)) was 1.135 3×10~6 conidia·mL~(-1).By infecting trials to H.xiaoi in a forest with the strain Ma12912,mortality of the treatment was extremely significant higher than the control,the correct mortality,the muscardine cadaver rate and the conidia content individual of H.xiaoi were 57.85%±1.19%,55.43%± 2.90% and (1.12±0.41)×10~8 conidia individual after 30 d,respectively,and the median lethal times (LT_(50)) was 16.56 d.The results suggest that the strain of Ma1291-2 has great application value.
Keywords:Hylobitelus xiaoi  Metarhizium anisopliae  pathogenicity  strain screening  
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