| 水体中硝基苯厌氧降解微生物的筛选及其降解特性研究 |
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| 引用本文： | 卢桂兰,郭观林,王世杰,谷庆宝,李发生.水体中硝基苯厌氧降解微生物的筛选及其降解特性研究[J].农业环境科学学报,2010,29(3). |
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| 作者姓名： | 卢桂兰 郭观林 王世杰 谷庆宝 李发生 |
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| 作者单位： | 1. 中国环境科学研究院土壤污染与控制研究室,北京,100012
2. 中国环境科学研究院土壤污染与控制研究室,北京,100012;北京科技大学土木与环境工程学院,北京,100083 |
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| 基金项目： | 全国土壤调查修复专项(NC5120801);;科技部国际合作专项(2008DFR90550);;院公益项目(GYK5120803) |
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| 摘 要： | 以硝基苯为碳源,采用间歇式曝气方式,经90d驯化培养,从处理硝基苯废水的生物活性污泥中分离得到13株高效降解菌,在厌氧环境条件分离纯化,筛选得到降解能力最强的MY4菌,并研究了其对不同浓度硝基苯的降解特性。结果表明,经16SrRNA鉴定,MY4菌属于兼性葡萄球菌属(Staphylococcus sp),其在104～107CFU·mL-1范围内对硝基苯的去除率没有显著差异,降解率均为65%,硝基苯的降解速率在3～5d期间达到最大。高浓度的硝基苯对微生物的降解过程存在抑制作用,随着浓度的增高,其降解半衰期明显延长。GC-MS分析表明,硝基苯经MY4菌株降解后的主要产物为苯胺。
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| 关 键 词： | 硝基苯 污水 厌氧微生物 生物降解 |
Screening and Biodegradation of Anaerobic Microorganisms for Nitrobenzene in Water |
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| LU Gui-lan,GUO Guan-lin,WANG Shi-jie,GU Qing-bao,LI Fa-sheng.Screening and Biodegradation of Anaerobic Microorganisms for Nitrobenzene in Water[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2010,29(3). |
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| Authors: | LU Gui-lan GUO Guan-lin WANG Shi-jie GU Qing-bao LI Fa-sheng |
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| Institution: | 1.Department of Soil Pollution Control/a>;Chinese Research Academy of Environmental Sciences/a>;Beijing100012/a>;China/a>;2.School of Civil & Environmental Engineering/a>;University of Science and Technology Beijing/a>;Beijing 100083/a>;China |
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| Abstract: | 13 high efficiency bacterium strains were obtained from the activated sludge in waste water contaminated with nitrobenzene after 90 days intermittent aeration. MY4 was screened out in anaerobic condition, which was identified to be the most efficient bacteria strain. Further experiments were conducted on the biodegradation characteristics of MY4 strain. Results indicated that MY4 was Staphylococcus sp based on the 16S rRNA analysis. No obvious distinctions were observed in the biodegradation of nitrobenzene... |
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| Keywords: | nitrobenzene wastewater anaerobic microorganism biodegradation |
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