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大叶冬青叶若干生化指标测定及抗菌活性研究
引用本文:李晓储,蒋继宏,方德兰,高甜惠,曹小迎,黄利斌.大叶冬青叶若干生化指标测定及抗菌活性研究[J].扬州大学学报(农业与生命科学版),2006,27(1):91-94.
作者姓名:李晓储  蒋继宏  方德兰  高甜惠  曹小迎  黄利斌
作者单位:1. 徐州师范大学,江苏省药用植物生物技术重点实验室,江苏,徐州,221116;江苏省林业科学研究院,江苏,南京,211153
2. 徐州师范大学,江苏省药用植物生物技术重点实验室,江苏,徐州,221116
3. 江苏省林业科学研究院,江苏,南京,211153
基金项目:江苏省农业三项工程项目(SX2001-0687),国家“十五”重大科技攻关项目(2002BA516A-07)
摘    要:对大叶冬青叶若干生化指标含量的测定结果表明:不同处理叶各生化指标含量存在明显差异,可溶性蛋白质含量,微波干燥叶与冷冻干燥叶相近,分别是鲜叶的46.3%和45.3%;超氧化物岐化酶含量,冷冻干燥叶与微波干燥叶分别是鲜叶的4.8和4.2倍。大叶冬青叶水层物总生物碱、总黄酮甙元对金黄色葡萄球菌和大肠杆菌均有较强的抑菌活性。大叶冬青既可作为绿化苗木种植,又可利用其叶作保健、药用产品进行深度开发。

关 键 词:大叶冬青  可溶性蛋白质  超氧化物岐化酶  抗菌活性
文章编号:1671-4652(2006)01-0091-04
修稿时间:2005年3月28日

Study on the biochemical indexes and antimicrobia activities of the Ilex latifolia leave
LI Xiao-chu,JIANG Ji-hong,FANG De-lan,GAO Tian-hui,CAO Xiao-ying,HUANG Li-bin.Study on the biochemical indexes and antimicrobia activities of the Ilex latifolia leave[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2006,27(1):91-94.
Authors:LI Xiao-chu  JIANG Ji-hong  FANG De-lan  GAO Tian-hui  CAO Xiao-ying  HUANG Li-bin
Abstract:By determining the health care constituents, the results showed the leaves of Ilex latifolia contained soluble proteins and SOD which are helpful to people. The contains of soluble proteins and SOD in different treatments showed distinctive differences. The soluble proteins from the leaves treated with microwave dry and freezed dry were 46. 3% and 45. 3% of fresh leaves from I. latifolia respectively. The contains of SOD in the leaves treated with microwave dry and freezed dry were 4. 8-fold and 4. 2-fold more than fresh leaves from 7. latifolia. The alkaloids, water layer constituents and total flavonoide aglycone showed strong antibacterial activity against Staphalococus aureus and Escherichia coli. The research work provided scientific basis for virescence seedling production and plant medicine development of I. latifolia.
Keywords:Ilex latifolia  soluble protein  SOD  antibacterial compounds
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