冬瓜和节瓜种质资源遗传多样性的RAPD分析

采用RAPD分子标记技术对41份冬瓜和节瓜种质资源进行遗传多样性分析。从455条随机引物中筛选出23条能产生稳定多态性扩增谱带的引物用于RAPD分析,共产生143条扩增谱带,其中多态性谱带81条,多态性检测率为56.6%,表明冬瓜和节瓜种质资源在分子水平上具有丰富的遗传多样性。用NTSYSpc软件处理RAPD数据,41份材料间的遗传相似系数在0.60～0.99之间。聚类分析结果可将供试材料分为6大类群9个亚类,主坐标分析可将供试材料分为6大类群10个亚类。两种分类方法的结果基本一致。

RAPD Analysis of Genetic Diversity of Wax Gourd and Chieh-qua Germplasm

RAPD analysis was applied to analysis the genetic diversities of 41 wax gourd（Benincasa hispida Cogn.）and chieh-qua（Benincasa hispida var. chieh-qua How）germplasm. 23 polymorphic primers screened out of 455 random primers were used and 143 reproducible bands were produced. Among them, 81 bands were polymorphic and polymorphism detection rate was 56.6 %. This indicated that genetic diversity of wax gourd and chieh-qua germplasm was very rich. RAPD data were analysized by NTSYSpc software, and results showed that the genetic similarity was among the range of 0.60 to 0.99. 41 germplasm were divided into 6 groups and 9 subgroups by clustering analysis, while 6 groups and 10 subgroups by the principal coordinates analysis. The results of germplasm classification according to the above two methods were generally the same.