| 狂犬病病毒Vero ERA株G蛋白333位点精氨酸突变株的构建 |
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| 引用本文： | 郭利,王喜军,冯娜,葛金英,杨松涛,夏咸柱,步志高.狂犬病病毒Vero ERA株G蛋白333位点精氨酸突变株的构建[J].中国畜牧兽医,2009,36(9):79-82. |
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| 作者姓名： | 郭利 王喜军 冯娜 葛金英 杨松涛 夏咸柱 步志高 |
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| 作者单位： | （1.吉林农业大学研究生院， 长春 130118； 2.军事医学科学院军事兽医研究所， 长春 130000； 3.中国农业科学院哈尔滨兽医研究所农业部兽医公共卫生重点开放实验室暨兽医生物技术国家重点实验室， 哈尔滨 150001） |
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| 基金项目： | 国家重点基础研究规划(978)项目:病毒变异与致病相关性的分子基础，国家科技部支撑计划项目:"狂犬病、血吸虫病等重要人畜共患病疫苗与诊断试剂的研制与开发"，国家高科技研究发展计划:"狂犬病传染源综合防控技术研究"，863计划:"家畜重要病毒病基因工程疫苗研究与创制"，国家自然科学基金重点项目""狂犬病病毒跨种间感染与传播机制研究" |
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| 摘 要： | 狂犬病病毒糖蛋白（G）在病毒的致病性中起着主要作用，其第333位点精氨酸（Arg）是决定病毒神经细胞侵嗜性的重要分子基础之一。为研制更加安全有效的狂犬病减毒活疫苗，本试验采用负链RNA病毒反向遗传学方法，在体外将狂犬病病毒Vero细胞适应株ERA糖蛋白G333位点精氨酸突变为谷氨酸，构建减毒株ERAGΔ，减弱了其潜在的毒力返强的危险。救获的ERAGΔ突变株在Vero细胞上表现出与ERA株亲本病毒相似的生长动力学特性。本研究成功构建并拯救出了狂犬病病毒ERA减毒疫苗株，为研制开发狂犬病病毒无毒疫苗提供了候选株。
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| 关 键 词： | 狂犬病病毒 反向遗传操作 糖蛋白G 人工突变 |
Rescue of Vero Cell Adapted Rabies Virus ERA Vaccine Strain with R333E Mutation in Glycoprotein |
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| GUO Li,WANG Xi-jun,FENG Na,GE Jin-ying,YANG Song-tao,XIA Xian-zhu,BU Zhig-ao.Rescue of Vero Cell Adapted Rabies Virus ERA Vaccine Strain with R333E Mutation in Glycoprotein[J].China Animal Husbandry & Veterinary Medicine,2009,36(9):79-82. |
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| Authors: | GUO Li WANG Xi-jun FENG Na GE Jin-ying YANG Song-tao XIA Xian-zhu BU Zhig-ao |
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| Institution: | (1.Graduate School, Jilin Agriculture University, Changchun 130118, China; 2.The Institute of Veterinary Medicine, Academy of Military Medical Science, Changchun 130000, China; 3.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China) |
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| Abstract: | The glycoprotein (G) of rabies virus (RV) is known to play a predominant role in the pathogenesis of rabies. And G333 (Arg) is one of molecular basis that decided neurocyte tropism in rabies virus. For developing new vaccine of rabies virus which is safer and has good immunogenicity, based on reverse genetics, the antigen site R333 of Glycoprotein, in the Vero cell adapted ERA vaccine strain, was mutated from R to E. And rescued the attenuated vaccine strain ERAGΔ successfully. TCID50 was between 10-4 and 10-5, in vitro growth the virus of ERAGΔ was reproduce after 24 hours, and the highest titer was in five days, then fall-off. The result shows the same typical grows kinetics with parents ERA in Vero cells. It was a substantial technique for the next research on attenuated vaccine. In this experiment, the attenuated vaccine strain ERAGΔ was rescued successfully, and provided a good candidate for vaccine. It has great value for next research and practical application. |
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| Keywords: | rabies virus reverse genetics glycoprotein mutation |
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