玉米籽粒淀粉含量的遗传效应分析

以玉米完整籽粒为试验材料,采用偏最小二乘回归法建立近红外反射光谱测定其淀粉含量的数学模型,光谱预处理结果表明,采用一阶导数+多元散射校正建立淀粉含量的校正模型效果最佳,外部验证结果表明,校正后模型预测结果与化学值之间的相关系数达0.924 0。用13个普通玉米自交系,按NCⅡ设计(6×7)研究玉米籽粒品质性状的遗传效应,结果表明:粗淀粉含量不同程度地体现为种子、母体和细胞质效应,而母体遗传效应和种子直接效应占主导,细胞质效应相对较小,遗传效应以母体加性和胚乳直接加性为主,存在显著的正向母体杂种优势。遗传效应预测值表明,铁7922、郑58是选育高淀粉品种的优良材料,同时说明以校正模型测定其含量结果准确、可靠。

Genetic Analysis of Seed Starch Content in Maize

The starch content of whole maize kernel was determined by Near Infrared Reflectance Spectroscopy.The math model was established by partial least square regression.The result of spectrum pretreatment indicated that the calibration model developed by first order derivative with multivariate scattering correction had the best effect for starch content.Exterior validation suggested that the correlation coefficient between the predicted NIRS value by Calibration model and the starch content obtained from chemical methods was 0.9240.Thirteen maize inbred lines were crossed in NCⅡdesign(6×7)to analyze genetic effects of seed quality properties.The results indicated that the crude starch content was controlled by seed,maternal and cytoplasmic genetic effects with different degrees,but the maternal and seed direct effects were dominant and the cytoplasmic genetic effect was correspondingly minor.Maternal and seed direct additive heretic effects were more important and significantly positive maternal heterosis was found.The predicted genetic effects showed that inbred lines Tie7922 and Zheng58 were better than other parents in breeding high starch varieties.It is very exact and credible to measure the starch content by the calibration model.