| 正交设计在灯盏花组织培养中的应用* |
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| 引用本文： | 黄平,王荔,杨艳琼,陈疏影,刘雅婷,杨凯,刘波.正交设计在灯盏花组织培养中的应用*[J].云南农业大学学报,2006,21(2):160-163. |
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| 作者姓名： | 黄平 王荔 杨艳琼 陈疏影 刘雅婷 杨凯 刘波 |
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| 作者单位： | 云南农业大学农学与生物技术学院,云南昆明650201 |
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| 摘 要： | 针对灯盏花自然繁殖率低下的情况,探讨了利用植物组织培养方法解决灯盏花生产中繁殖的问题。按正交实验设计的原则,摸索出建立灯盏花组织培养技术体系的最佳条件:不定芽诱导的培养基是MS+6-BA 1.0mg/L+NAA 0.1mg/L,生根培养基是MS+ NAA 0.5mg/L+ IAA 0.2mg/L。
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| 关 键 词： | 灯盏花 组织培养 正交设计 |
| 文章编号： | 1004-390X（2006）02-0160-04 |
| 收稿时间： | 2005-11-03 |
| 修稿时间： | 2005-11-03 |
Application of Orthogonal Design in Tissur Culture of Erigerm breviscapus(Vant.)Hand.-Mazz |
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| HUANG Ping,WANG Li,YANG Yan-qiong,CHEN Shu-ying,LIU Ya-tin,YANG Kai,LIU Bo.Application of Orthogonal Design in Tissur Culture of Erigerm breviscapus(Vant.)Hand.-Mazz[J].Journal of Yunnan Agricultural University,2006,21(2):160-163. |
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| Authors: | HUANG Ping WANG Li YANG Yan-qiong CHEN Shu-ying LIU Ya-tin YANG Kai LIU Bo |
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| Institution: | College of Agronomy and Biotechnology, Y A U, Kunming 650201, China |
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| Abstract: | Because the fruiting rate and germinative percentage of Erigerm breviscapus(Vant.)Hand.-Mazz are lower, tissue culture was explored to solve the propagating problem in the production. By using the orthogonal experiment design, the experimental result shows that the best culture medium of inducing adventitious bud was MS+6-BA 1.0mg/L+NAA 0.1mg/L,the best culture medium of root was NAA 0.5mg/L+ IAA 0.2mg/L |
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| Keywords: | Erigerm breviscapus(Vant )Hand -Mazz tissue culture orthogonal design |
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