脱毒银合欢饲喂小白鼠的安全利用研究

选取平均体重18~21 g左右,雌雄各半的N IH系小白鼠50只,随机分为5组,每组10只,进行5周饲养试验。A组为对照组,饲喂小白鼠基础日粮。B、C、D、E组为试验组,在小白鼠基础日粮上分别添加20%、30%的晒干银合欢叶粉和20%、30%经过1.2%F eC l3处理的银合欢叶粉。试验结束时,全部扑杀,采血检查血常规血清,剖检,取下脏器称重,计算心、肝、脾、肺、肾的脏器系数。结果表明:所有银合欢叶粉替代部分基础日粮饲喂小白鼠,适口性好,小白鼠正常采食。用经过1.2%F eC l3处理的银合欢叶粉替代20%的基础日粮饲喂小鼠,小白鼠在最后平均体重、平均日增重、料重比方面相近于对照组(P>0.05),优于用经过F eC l3处理的银合欢叶粉替代30%的基础日粮组。各组的心、肝、脾、肺、肾系数之间无显著差异(P>0.05),内脏器官正常。B、C组的血红蛋白(HGB)、红细胞(RBC)、白细胞(W BC)与对照组差异显著(P<0.05)。D、E组的血红蛋白(HGB)、红细胞(RBC)、白细胞(W BC)、淋巴细胞(LYM PH)与对照组没有显著差异(P>0.05)。各组的尿素氮(BUN)、肌酐(CRE)、谷丙转氨酶(ALT)、谷草转氨酶(A ST)均无显著差异(P>0.05)。

The Secure Research of Diet with Leucaena Leucocephala on the Mice

50 NIH mice were randomly divided into 5 groups(10 mice each group) by sex and weight,i.e,group ABCDE respectively.They were offered the control ration,and in other groups a portion of the control ration was replaced by Leucaena Leucocephala(LL) treated with 1.2% FeCl_3 or untreated,i.e.,20% LL,30% LL,20% treated LL,and 30% treated LL ration in a 5 weeks feeding trial. The result showed that: the mice enjoyed intaking LL;there were no significant difference in the last body weightthe daily gain and the feed/gain ratio between the test group of replaced 20% treated LL and the control group(P>0.05).HGBRBCWBC of BC groups were different significantly compared with those of control group(P<0.05).There was no significant difference in HGBRBCWBC and LYMPH between the DE groups and the control group(P>0.05).The experiment also indicated that all the blood biochemical parameter and the organ coefficients were not different significantly(P>0.05).