| Research on protoplast preparation and culture in Gladiolus hybridus Hort. |
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| 作者姓名： | ZHANG Ying GONG Sufang WANG Jingang and CHE Daidi* College of Horticulture Northeast Agricultural University Harbin China |
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| 作者单位： | ZHANG Ying，GONG Sufang，WANG Jingang，and CHE Daidi* College of Horticulture，Northeast Agricultural University，Harbin 150030，China |
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| 基金项目： | Supported by Heilongjiang Natural Sciences of Fundation （C2004-01 ） |
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| 摘 要： | Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions: 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L~(-1) mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L~(-1) mannitol, 1.0 mg· L~(-1) 2, 4-D, 0.2 mg· L~(-1) NAA and 0.2 mg· L~(-1) KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times.
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| 关 键 词： | 唐菖蒲 原生质体培养 品种改良 遗传转化 |
| 文章编号： | 1006-8104（2007）-01-0005-04 |
| 收稿时间： | 2006-01-15 |
Research on protoplast preparation and culture in Gladiolus hybridus Hort. |
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| ZHANG Ying,GONG Sufang,WANG Jingang,and CHE Daidi* College of Horticulture,Northeast Agricultural University,Harbin ,China.Research on protoplast preparation and culture in Gladiolus hybridus Hort.[J].Journal of Northeast Agricultural University,2007,14(1):5-8. |
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| Authors: | ZHANG Ying GONG Sufang WANG Jingang CHE Daidi |
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| Abstract: | Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions: 1.5％ cellulase Onzuka RS, 0.5％ pectinase Y-23, 0.6 mol· L-1 mannitol, pH 5.8, digestion time 2 h, and temperature 27 ℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L-1 mannitol, 1.0 mg· L-1 2, 4-D, 0.2 mg· L-1 NAA and 0.2 mg· L-1 KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times. |
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| Keywords: | Gladiolus hybridus aseptic leaves protoplast culture |
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