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Effectiveness of exogenous DNA transfer to chicken embryo cells in vitro and in vivo using retroviral vectors
Authors:N. A. Volkova  A. O. Tulyakova  L. A. Volkova  N. A. Zinov’eva  L. K. Ernst  E. K. Tomgorova  N. S. Lotsmanova
Affiliation:(1) All-Russian State Animal Husbandry Research Institute, Moscow oblast, 142132, Russia
Abstract:The frequency of genetic transformation of target cells in a culture is established to be from 9 × 10?4 to 3 × 10?3. A transgenic rooster with integration and expression of the lacZ gene in heart, liver, intestine, and testis cells is produced.
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