| 香蕉ACC合成酶cDNA的PCR扩增及序列测定 |
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| 引用本文： | 金志强 彭世清. 香蕉ACC合成酶cDNA的PCR扩增及序列测定[J]. 热带作物学报, 1998, 19(1): 48-51 |
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| 作者姓名： | 金志强 彭世清 |
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| 作者单位： | 中国热带农业科学院热带作物生物技术国家重点实验室 |
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| 摘 要： | 从香蕉果实中提取总ＲＮＡ，经反转录成ｃＤＮＡ第一链，根据已知植物ＡＣＣ合成酶的ｃＤＮＡ及其所编码的氨基酸序列合成引物，经ＰＣＲ扩增香蕉ｃＤＮＡ得到一长约１．１Ｋｂ的产物，对该序列测定结果表明，该ｃＤＮＡ所编码的氨基酸序列包括了ＡＣＣ合成酶８个高度保守区中的７个，并且包括该酶的活性中心。
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| 关 键 词： | 香蕉 ACC合成酶 PCR CONA 序列测定 |
Amplification and sequencing of a cDNA Encoding ACC Synthase of Banana |
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| Jin Zhiqiang, Peng Shiqing, Shao Hanshuang, Kong Deqian, Zheng Xueqin. Amplification and sequencing of a cDNA Encoding ACC Synthase of Banana[J]. Chinese Journal of Tropical Crops, 1998, 19(1): 48-51 |
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| Authors: | Jin Zhiqiang Peng Shiqing Shao Hanshuang Kong Deqian Zheng Xueqin |
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| Abstract: | A cDNA from banana fruit was amplified with PCR and sequenced,resulting ina PCR product of 1098 bp long. Its deduced amino acids sequence showed it containedseven of the eight highly conserved regions(HCR)of ACC synthase and the similar aminoacids were over 95% in HCR,which is agreeable to the results from other plants. |
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| Keywords: | Banana ACC synthase PCR |
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