| CBF调节子在水稻品种日本晴和93 11低温驯化过程中的差异调控机制 |
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| 引用本文： | 潘孝武,黎用朝,李小湘,刘文强,闵军,陆婷婷,盛新年,谭江. CBF调节子在水稻品种日本晴和93 11低温驯化过程中的差异调控机制[J]. 中国水稻科学, 2012, 26(5): 521-528. DOI: 10.3969/j.issn.1001 7216.2012.05.002 |
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| 作者姓名： | 潘孝武 黎用朝 李小湘 刘文强 闵军 陆婷婷 盛新年 谭江 |
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| 作者单位： | 湖南省水稻研究所,湖南长沙,410125 |
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| 基金项目： | 长江中下游突发性灾害应急防控技术研究与集成示范(2012BAD20B05-01-1);国家现代农业产业技术体系建设专项(nycytx-001);转基因生物新品种培育科技重点项目(2009ZX08001-009B) |
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| 摘 要： | 利用拟南芥DREB1蛋白序列在水稻全基因组水平进行TBLASTN，共检索到9个水稻同源基因。其中，有3个基因串联排列在第9染色体上，命名为OsCBF1、OsCBF2、OsCBF3。它们编码的蛋白在保守区域内与DREB1的相似性较高。低温驯化能增强水稻幼苗的耐冷性，减少叶片的离子渗漏，但粳稻品种日本晴和籼稻品种93 11之间存在差异。OsCBF1~OsCBF3基因在低温驯化过程中诱导表达，在93 11中的表达量比在日本晴中的更高。CBF候选靶标基因OsP5CS在两个水稻品种都得到诱导表达，但是OsLIP5和OsLIP9只在93 11中上调表达，而在日本晴中表达量变化不明显。OsCBF1~OsCBF3的启动子序列在日本晴与93 11之间存在多态性，93 11的启动子序列中包含更多的MYC顺式作用元件。
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| 关 键 词： | 水稻 低温驯化 基因表达 启动子分析 |
| 收稿时间： | 2012-02-26; |
Differential Regulatory Mechanism of CBF Regulon Between Nipponbare(japonica) and 93-11(indica) During Cold Acclimation |
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| PAN Xiao-wu , LI Yong-chao , LI Xiao-xiang , LIU Wen-qiang , MIN Jun , LU Ting-ting , SHENG Xin-nian , TAN Jiang. Differential Regulatory Mechanism of CBF Regulon Between Nipponbare(japonica) and 93-11(indica) During Cold Acclimation[J]. Chinese Journal of Rice Science, 2012, 26(5): 521-528. DOI: 10.3969/j.issn.1001 7216.2012.05.002 |
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| Authors: | PAN Xiao-wu LI Yong-chao LI Xiao-xiang LIU Wen-qiang MIN Jun LU Ting-ting SHENG Xin-nian TAN Jiang |
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| Affiliation: | (Rice Research Institute of Hunan,Changsha 410125,China;) |
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| Abstract: | Nine CBF/DREB1 homology genes in rice were obtained by BLAST search in the NCBI database,which shares conserved amino acid sequences with DREB1 protein in Arabidopsis.Three CBF genes organized in tandem,named OsCBF1,OsCBF2,OsCBF3,showed a transient induction in the process of cold acclimation,but much stronger in 93-11 compared with that in Nipponbare.The candidate downstream genes OsLIP5 and OsLIP9 were induced in 93-11 but not in Nipponbare.However,the expression of OsP5CS was induced in both varieties.The differential expression of CBF regulon might be caused by polymorphisms within promoter sequences between these two rice varieties. |
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| Keywords: | rice cold acclimation gene expression promoter analysis |
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