| 秦艽体细胞胚胎发生及植株再生的研究 |
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| 引用本文： | 魏莉霞,漆燕玲.秦艽体细胞胚胎发生及植株再生的研究[J].农业科技通讯,2010(7):33-37. |
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| 作者姓名： | 魏莉霞 漆燕玲 |
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| 作者单位： | 甘肃省农业科学院啤酒原料研究所特作研究室,甘肃兰州,730070 |
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| 基金项目： | 甘肃省名贵中药材秦艽、半夏品种改良研究(甘肃省农业生物技术研究与应用开发项目) |
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| 摘 要： | 以秦艽子叶与胚轴为外植体,诱导秦艽体细胞胚胎发生并建立植株再生体系。结果表明：不同外植体以及植物激素浓度和种类对体细胞胚胎发生均有一定影响,其中秦艽的子叶胚性愈伤组织的诱导率最佳激素组合为2,4-D1.0mg/L,6-BA0.5mg/L,其诱导率为61.3%,对胚轴胚性愈伤组织诱导最佳激素为2,4-D1.0mg/L,其诱导率为42.3%。将胚性愈伤组织转接至MS培养基,都可产生再生植株。
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| 关 键 词： | 秦艽 愈伤组织诱导 植株再生 |
Study on Somatic Embryogenesis and Plant Regeneration of Gentiana macrophylla Pall |
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| Authors: | WEI Li-xia QI Yan-ling |
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| Institution: | (Special Crops Laboratory of Beer Material Institute, Gansu Academy of Agricultural Sciences Lanzhou Gansu 730070) |
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| Abstract: | Cotyledon and hypocotyl explants of GentianamacrophyUa Pall were cultured to induce so- matic embryogenesis and then the somatic embryos were developed into seedlings.The results revealed that diferent explants and plant growth regulation agents had different effects on somatic embryogenesis, and the best medium to induce somatic embryogenesis of Gentiana macrophylla Pall Cotyledon was MS medium containing 1.0mg/L2,4-D and 0.Smg/L6-BA, the intuctivity is 61.3%, to hypocotyl explants, the best medium was MS medium containing 1.0mg/L2,4-D , the intuctivity is 42.3%.The somatic embryos were germinated and could developed into plantlets by culturing on MS medium. |
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| Keywords: | Gentiana macrophylla Pall callus culture plant regeneration |
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