| PCR—DGGE技术在我国冷水鱼微生态学研究中的应用前景 |
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| 引用本文： | 穆贵强,卢彤岩,刘红柏,王获. PCR—DGGE技术在我国冷水鱼微生态学研究中的应用前景[J]. 鲑鳟渔业, 2013, 0(6): 58-62 |
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| 作者姓名： | 穆贵强 卢彤岩 刘红柏 王获 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 |
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| 基金项目： | “十二五”国家科技支撑计划（2012BAD2580204）. |
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| 摘 要： | PCR-DGGE技术已经发展成为研究微生物的群落结构和遗传多样性的有效工具。在世界水产养殖业的养殖系统微生态学、肠道细菌微生态学和益生菌研究中都取得了一系列的应用成果，而在我国冷水鱼的研究中应用还较少。本文介绍了PCR-DGGE技术的特性及其在世界水产养殖业研究中的应用，分析了其在我国冷水鱼微生态学研究中的应用前景。
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| 关 键 词： | 变性梯度凝胶电泳 冷水鱼微生态学 应用前景 |
The Application and Research Prospects of PCR-DGGE in Coldwater Fishes Microecology in China |
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| Affiliation: | MU Gui-qiang, LU Tong-yan, LIU Hong-bai, WANG Di (HeilonNiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China) |
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| Abstract: | PCR-DGGE （polymerase chain reaction-density gradient gel electrophoresis） technique as an effective tool for the study of microbial community structure and genetic diversity has made a good progress in application of world aquaculture system, especially micro-ecology, intestinal bacterial micro-ecology and probiotics research. However, the application of PCR-DGGE in coldwater fishes is rarely reported in China. The technological characteristics and role of PCR-DGGE in world aquaculture research are described and the research prospects of its application in coldwater fish in China are discussed. |
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| Keywords: | PCR-DGGE micro-ecology in coldwater fish application prospect |
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