色素腺体和棉酚对陆地棉茎尖培养的影响

本研究以陆地棉三对色素腺体近等基因系为材料,研究色素腺体和棉酚对棉花茎尖培养的影响.结果表明,外植体的色素腺体和棉酚性状对于棉花茎尖培养有较大的影响,有色素腺体棉的茎尖比对应的无色素腺体近等基因系更易诱导产生不定根和再生植株.无色素腺体棉根尖的不定根出生时间晚于对应的有色素腺体近等基因系7天左右.棉酚试验

The Effects of Pigment Glands and Gossypol on the Stem Tip Culture of Upland Cot ton (Gossypium hirsutum L. )

The effects of pigment glands and gossypol on the stem tip culture of upland cotton were studied, using three pairs of glanded and glandless upland c otton isogenic lines as materials. The results showed that the pigment glands a nd goss ypol characters in the explants had great effect on the cotton stem tip culture, the inducing of adventitious roots and plant regeneration of the glanded cotton were much easier than that of their glandless cotton isogenic lines The emerge nc e time of adventitious roots for glandless cotton stem tip cultured in vitro was late about a week, comparing with their glanded isogenic lines The extra g ossypo l in the medium had no significant effect on the stem tip culture of glanded cot ton, but enhance effect on the glandless cotton isogenic lines The medium with 0.1 mg/ L of extra gossypol was the best one for glandless cotton stem tip culture, on w hich the inducing rate of adventitious roots and plant regeneration of the gland less cotton stem tip culture were same, or even better than that of their glande d cotton isogenic lines on the medium without extra gossypol. However, too much gossypol in vitro was unfavorable for the cotton stem tip culture, when the concentration of the in vitro gossypol in the medium was higher than 0.20 mg /L, the adventitious root inducing, as well as the growth and development of the plant regeneration from the stem tip culture of both glanded and glandless cott on were inhibited seriously.