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| 奶牛乳腺炎大肠杆菌PCR的检测 | |
| 引用本文： | 任可,刘月焕,王凤龙,步卫东,林健,王金玲,杨龙峰. 奶牛乳腺炎大肠杆菌PCR的检测[J]. 内蒙古农业大学学报(自然科学版), 2006, 27(4): 58-61 |
| 作者姓名： | 任可 刘月焕 王凤龙 步卫东 林健 王金玲 杨龙峰 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018 2. 北京市农林科学院畜牧所,北京,100097 3. 中国农业大学,北京,100094 |
| 摘 要： | 通过对内蒙古呼和浩特地区分离3株奶牛乳腺炎大肠杆菌16S-23S rRNA间隔区的PCR检测、基因测序和序列比较分析,结果表明:分离菌株12C与参考菌株的同源率为99.9%,DG-25A和DG-23A与参考菌株的同源率为98.9%,3株分离菌株的同源性高。应用PCR检测奶牛乳腺炎大肠杆菌的方法简便、快速、特异,该方法也可广泛用于乳房炎其他病原菌的检测。 |
| 关 键 词： | 奶牛乳房炎 大肠杆菌 16S-23SrRNA间隔区 PCR |
| 文章编号： | 1009-3575(2006)04-0058-04 |
| 收稿时间： | 2006-09-07 |
| 修稿时间： | 2006-09-07 |
IDENTIFICATION OF ESCHERICHIA COLI OF COW MASTITIS USING PCR |
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| REN Ke,LIU Yue-huan,WANG Feng-long,BU Wei-dong,LIN Jian,WANG Jin-ling,YANG Long-feng. IDENTIFICATION OF ESCHERICHIA COLI OF COW MASTITIS USING PCR[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2006, 27(4): 58-61 | |
| Authors: | REN Ke LIU Yue-huan WANG Feng-long BU Wei-dong LIN Jian WANG Jin-ling YANG Long-feng |
| Affiliation: | 1. Inner Mongolia Agricultural University,Huhhot 010018, China; 2. Institute of Animal Husbandry and Veterinary Science, Beijing Academy of Agriculture and Forestry Science,Bering 100097 China; 3. China Agriculture University ,Beijing 100094 , China |
| Abstract: | Three isolates obtained from Huhhot region in Inner Mongolia were investigated by PCR,sequences and analyses with oligonucleotide primers designed according to species-specific parts of the 16S-23S rRNA spacer region of the species.The results showed that the sequence homology was 99.9% between isolate 12C and reference strain and it was 98.9% between strain DG25A,DG23A and reference strain.There was higher homology within three isolates.The PCR amplification of speciesspecific sequences of Escherichia coli in the study offers a rapid,specific and sensitivity method to diagnose the mastitis. |
| Keywords: | Polymerase chain reaction(PCR) mastitis Escherichia coli 16S23S rRNA spacer regions |
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