| 转基因SOD，POD高表达烟草幼苗抗热性研究* |
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| 引用本文： | 刘明求,刘齐元,丁小维,侯思名,刘飞虎. 转基因SOD，POD高表达烟草幼苗抗热性研究*[J]. 云南农业大学学报(自然科学版), 2005, 20(5): 620-623 |
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| 作者姓名： | 刘明求 刘齐元 丁小维 侯思名 刘飞虎 |
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| 作者单位： | 云南大学生命科学学院 云南昆明650091(刘明求，刘齐元，丁小维，侯思名)，云南大学生命科学学院 云南昆明650091(刘飞虎) |
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| 基金项目： | 云南省人才基金资助项目 |
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| 摘 要： | 生理高温（38±1）℃ 处理转基因烟草品系及非转基因品系的幼苗，测定其SOD,丙二醛、可溶性糖含量、电导率和生物量等指标。结果表明，转基因品系SOD的活性更强，丙二醛的含量更低，生物量受到的影响更小。由此认为，转基因烟草品系幼苗比非转基因品系的抗热性更强，其中转基因POD高表达烟草品系的抗热性最强。研究结果表明通过转基因方法提高植物的抗热性是可行的。
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| 关 键 词： | 生理高温 转基因烟草 抗热性 SOD POD |
| 文章编号： | 1004-390X（2005）05-0620-04 |
| 收稿时间： | 2005-03-13 |
| 修稿时间： | 2005-03-13 |
Heat Resistance Increases in Seedlings of Transgenic Tobacco Lines with SOD, POD Genes |
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| LIU Ming-qiu,LIU Qi-yuan,DING Xiao-wei,HOU Si-ming,LIU Fei-hu. Heat Resistance Increases in Seedlings of Transgenic Tobacco Lines with SOD, POD Genes[J]. Journal of Yunnan Agricultural University, 2005, 20(5): 620-623 |
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| Authors: | LIU Ming-qiu LIU Qi-yuan DING Xiao-wei HOU Si-ming LIU Fei-hu |
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| Affiliation: | （College of Life Sciences, Yunnan University, Kunming 650091, China） |
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| Abstract: | Seedlings of transgenic tobacco and non-transgenic tobacco lines were treated with physiological high temperature（38±1） ℃ for 3 d or 6 d. SOD activity, malondialdehyde content, soluble sugar content, electrical conductivity and biomass per plant were measured . While treated with physiological high temperature, SOD activities and biomass per plant were higher, malondialdehyde contents were lower in seedlings of transgenic tobacco lines than that in the non-transgenic line. The results indicated that heat resistance of transgenic tobacco lines was higher than that of the non-transgenic line. The line of transgenic tobacco with POD genes had the highest heat resistance. So it is possible to increase the heat resistance of plant by transgenic method. |
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| Keywords: | physiological high temperature transgenic tobacco heat resistance SOD POD |
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