| 柞蚕抗菌肽D转基因酵母的培养及表达产物的分析 |
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| 引用本文： | 马礼金,庄楚雄,黄自然. 柞蚕抗菌肽D转基因酵母的培养及表达产物的分析[J]. 蚕业科学, 1995, 0(1) |
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| 作者姓名： | 马礼金 庄楚雄 黄自然 |
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| 作者单位： | 华南农业大学 |
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| 摘 要： | 采用碱金属法（醋酸锂盐），以大肠杆菌－酵母穿梭质粒表达载体在酵母中表达人工合成的柞蚕抗菌肽Ｄ基因。用电泳及抗菌肽活性检测法对表达产物进行分析。同时还研究了转基因酵母的发酵条件，如温度、ｐＨ及营养等对酵母表达的影响。试验结果表明转基因酵母表达产物为１．１４ｍｇ／Ｌ培养液，杀菌活力为０．２１６单位。
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| 关 键 词： | 柞蚕，抗菌肽D基因，酵母 |
IMPROVED CULTURE METHOD FOR TRANSFER RING ANTIBACTERIAL PEPTIDE GENE FROM CHINESE OAK SILKWORM INTO YEAST AND IDENTIFICATION OF ITS EXPRESSED PRODUCTS |
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| Ma Li-jin Zhuang Chu-xiong Huang Zi-ran. IMPROVED CULTURE METHOD FOR TRANSFER RING ANTIBACTERIAL PEPTIDE GENE FROM CHINESE OAK SILKWORM INTO YEAST AND IDENTIFICATION OF ITS EXPRESSED PRODUCTS[J]. Acta Sericologica Sinica, 1995, 0(1) |
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| Authors: | Ma Li-jin Zhuang Chu-xiong Huang Zi-ran |
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| Abstract: | A synthetic antibacterial peptide gene(APG) from Chinese oak sikworm,Anther-aea pernyi was recombined with a shuttle plasmid pCL WA2 containing an alpha-factorpromotor and transferred into yeast AB103.The cells of yeast grew well under 18-30℃,pH 5.0 in YEPD medium.The expressed products were identified by electrophore-sis with detection of antibacterial activity.The expressed peptide has a molecular weightof 4000 daltons.The yield of product reached a level of 1.14mg/L YEPD and its activityunit is about 0.21 U. |
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| Keywords: | Antheraea pernyi Antibacterial peptide gene Yeast |
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