不同保藏温度及光照条件对北冬虫夏草菌种质量的影响

本实验模拟常见自然条件，以白昼散射光、夜晚黑暗，10/5℃、15/10℃、20/15℃、25/20℃等不同昼夜温度，30天、60天、90天等不同保藏时间为实验组，以4℃恒温、昼夜黑暗及30天、60天、90天为对照组，比较观察各菌种在平板培养基上萌发速度、菌丝生长状况、菌落形态及角变率、转扩液体菌种、栽培出草等情况。结果表明，菌种质量保藏较好的条件依次为10/5℃ 30天>15/10℃ 30天>4℃（黑暗）30天>20/15℃ 30天>4℃（黑暗）60天>10/5℃ 60天>25/20℃ 30天；说明一定条件下采用白昼散射光、昼夜10/5℃及15/10℃、保藏30天优于传统冰箱保藏。

Impact of different preservation temperature and light conditions on the quality of North Cordyceps species

Common natural conditions were simulated in this experiment.Scattered light to day,night,dark,10/5℃,15/10℃,20/15℃,25/20℃,30 d,60 d and 90 d preservation time for the different experimental groups were used as experiment teams.4℃ temperature,day and night dark and 30 d,60 d,90 d for the control group were used as control team,the bacteria in the plate medium speed germination,hyphal growth conditions,colony morphology and angular variability,turn expanded liquid strain,cultivated the grass,were observe.The results showed that strains the quality of the conditions were better preserved 10/5℃ 30 d15/10 ℃ 30 d4 ℃（dark） 30 d20/15 ℃ 30 d 4 ℃（dark） 60 d 10/5 ℃ 60 d 25/20 ℃ 30 d.That under certain conditions,the scattered light by day,day and night 10/5℃ and 15/10℃,30 d preservation than traditional preservation refrigerator.