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| 从干种子中快速获取高质量DNA的高盐提取方法 | |
| 引用本文： | 张富丽 陶李 佟洪金 刘勇 宋君 尹全 李忆 代晓航 雷绍荣 游米沙. 从干种子中快速获取高质量DNA的高盐提取方法[J]. 中国农学通报, 2011, 27(24): 98-102 |
| 作者姓名： | 张富丽 陶李 佟洪金 刘勇 宋君 尹全 李忆 代晓航 雷绍荣 游米沙 |
| 作者单位： | 1. 四川省农业科学院分析测试中心,成都,610066 2. 四川省环境保护科学研究院,成都,610064 3. 四川省农业科学院分析测试中心,成都610066;四川省农业科学院植保所,成都610066 4. 四川民族学院,四川康定,626001 |
| 基金项目： | 农业部转基因重大专项“转基因生物抽样和精准检测技术”(2008ZX08012-001),农业部“转基因生物安全评价共性技术”(2011ZX08011-006) |
| 摘 要： | 为寻求一种从植物干种子中简单快速提取高质量基因组DNA的有效方法进行本研究。将种子打磨成粉，取100 mg粉末加入高盐提取液抽提基因组DNA。采用超微量分光光度计检测、PCR及酶切的方法检验DNA的得率和质量。从100 mg 7种常见作物种子粉末中可以得到619.67~1811.21 ng基因组DNA，A260/A280比值在1.87~2.07之间，其纯度和质量适合进行PCR及酶切分析。通过普通PCR方法分别对7种作物的特异性内源基因片段进行扩增，结果均能扩增到明显的目的条带。用EcoRV和HindIII两种核酸内切酶能对所得的DNA充分酶切。结果表明本方法能快速地从干种子中提取到高质量的DNA。 |
| 关 键 词： | 小麦 小麦 航天诱变 醇溶蛋白 SRAP |
| 收稿时间： | 2011-04-26 |
| 修稿时间： | 2011-06-16 |
A Method of Simple and Rapid Salt-extraction of High Quality Genomic DNA from Plant Seeds |
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| Li Tao Hong-Jin Tong Quan Yin Xiao-Hang Dai Shao-Rong Lei Mi-Sha You. A Method of Simple and Rapid Salt-extraction of High Quality Genomic DNA from Plant Seeds[J]. Chinese Agricultural Science Bulletin, 2011, 27(24): 98-102 | |
| Authors: | Li Tao Hong-Jin Tong Quan Yin Xiao-Hang Dai Shao-Rong Lei Mi-Sha You |
| Affiliation: | Zhang Fuli 1,Tao Li 1,Tong Hongjin 2,Liu Yong 1,3,Song Jun 1,Yin Quan 1,Li Yi 4,Dai Xiaohang 1,Lei Shaorong 1,You Misha 1(1 Analysis and Determination Center,Sichuan Academy of Agricultural Sciences,Chengdu 610066,2 Sichuan Research Academy of Environmental Protection Science,Chengdu 610064,3 Plant Protection Research Institute,4 Sichuan University for Nationalities,Kangding Sichuan 626001) |
| Abstract: | To find a new simple,fast and high quality genomic DNA extraction technique from dried seeds,this experiment was carried out.The seeds were ground to powder.100 mg powder of seeds was put in an eppendorf tube and the genomic DNA was extracted by our method,respectively.The final eluate was detected by ultramicro UV/Vis spectrophotometer.The purity and quality of the genomic DNA extracted was confirmed by PCR and enzyme digestion analysis.619.67 to 1811.21 ng of genomic DNA was extracted from 100 mg dried se... |
| Keywords: | genomic DNA polymerase chain reaction high salt-extraction dried seed |
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