| 棉花黄萎病拮抗细菌B.velezensis6-61拮抗蛋白发酵条件的优化 |
| |
| 引用本文： | 李红亚,李术娜,朱宝成.棉花黄萎病拮抗细菌B.velezensis6-61拮抗蛋白发酵条件的优化[J].中国农学通报,2011,27(21):93-99. |
| |
| 作者姓名： | 李红亚 李术娜 朱宝成 |
| |
| 作者单位： | 河北农业大学生命科学学院,河北保定,071001 |
| |
| 基金项目： | 河北省自然科学基金项目“棉花黄萎病拮抗细菌的筛选及抗菌蛋白的分离”(398152) |
| |
| 摘 要： | 为了提高棉花黄萎病拮抗细菌B.velezensis 6-61活性物质的产量,为进一步开展6-61菌株的后续研究奠定基础,对6-61菌株产生的活性物质的性质进行了分析,并对其发酵条件进行了优化研究.采用有机溶剂萃取、硫酸铵沉淀对菌株6-61发酵液进行处理并检测抑茵活性,初步确定了该拮抗物质为蛋白质.采用单因素试验对6-6...
|
| 关 键 词： | 大丽轮枝菌 拮抗茵 发酵条件 优化 |
| 收稿时间： | 2011/2/15 0:00:00 |
| 修稿时间： | 6/2/2011 12:00:00 AM |
Fermentation Conditions Optimization of Antagonistic Bacteria B. velezensis 6-61 Against Verticillium dahliae Kleb |
| |
| Li Hongya,Li Shuna,Zhu Baocheng.Fermentation Conditions Optimization of Antagonistic Bacteria B. velezensis 6-61 Against Verticillium dahliae Kleb[J].Chinese Agricultural Science Bulletin,2011,27(21):93-99. |
| |
| Authors: | Li Hongya Li Shuna Zhu Baocheng |
| |
| Institution: | Li Hongya,Li Shuna,Zhu Baocheng(College of Life Science,Hebei Agricultural University,Baoding 071001) |
| |
| Abstract: | In order to improve the yield of antagonistic substance produced by B.velezensis 6-61 which has antagonistic activity against Verticillium dahliae Kleb,the analysis of the properties of antagonistic substance and fermentation conditions optimization of stain 6-61 were studied to provide a basis for further study of the antagonistic mechanism.First,the fermented broth of strain 6-61 was treated by two following methods:extraction with organic solvent and precipitation by ammonium sulfate respectively.The res... |
| |
| Keywords: | Verticillium dahliae Kleb antagonistic bacteria optimization fermentation conditions |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
