核桃亲子鉴定方法的建立

 【目的】针对核桃育种中父本不清、亲本混淆问题，建立简便快捷高效的核桃亲本鉴定方法，也为其它林木和果树品种亲本鉴定提供参考。【方法】以‘鲁果2号’为鉴定材料，采用表型性状和SSR标记的方法对‘鲁果2号’及其可能的亲本进行亲子分析。【结果】表型性状方法统计的8对坚果性状在各品种间差异很小，不能鉴定出子代亲本；SSR分型检测出疑似亲本中只有‘上宋6号’和‘鲁香’与子代‘鲁果2号’的等位基因遗传符合孟德尔遗传规律，其累积RCP（亲子关系概率）为99.997%，‘slz-40’与已知亲本鉴定结果与实际相符。【结论】‘上宋6号’和‘鲁香’是‘鲁果2号’的亲本，SSR分型技术较好地鉴别出了‘鲁果2号’的亲本，为其它林木和果树品种寻找和鉴别亲本，提供有效手段。

Establishment of Walnut Paternity Test

【Objective】A simple, rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding ,which provided reference for other varieties. 【Method】'Luguo-2' was used as material. Phenotypic traits and SSR markers were conducted to analyse parentage relationship between 'Luguo-2' and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results. In genotype results, the heredity of 'Shangsong-6', 'Luxiang' and 'Luguo-2' accords with Mendelian genetic regularity, there may be a paternity. PI and RCP wre analyzed by using the results of 10 SSR primer pairs, and the cumulative RCP was 99.997%. In addition, the appraisal result between ‘Slz-40’ and its known parent was consistent with the reality. 【Conclusion】'Shangsong-6' and 'Luxiang' were the parents of 'Luguo-2' with SSR clustering results supporting the conclusion. The parents of 'Luguo-2' were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.