首页 | 本学科首页   官方微博 | 高级检索  
     

核桃亲子鉴定方法的建立
引用本文:周贝贝,马庆国,王滑,王伟,张美勇,裴东. 核桃亲子鉴定方法的建立[J]. 中国农业科学, 2011, 44(20): 4258-4264. DOI: 10.3864/j.issn.0578-1752.2011.20.013
作者姓名:周贝贝  马庆国  王滑  王伟  张美勇  裴东
作者单位:1.中国林业科学研究院林业研究所;2.山东省果树研究所
基金项目:国家林业公益性行业科研专项经费项目,国家林业局重点项目
摘    要: 【目的】针对核桃育种中父本不清、亲本混淆问题,建立简便快捷高效的核桃亲本鉴定方法,也为其它林木和果树品种亲本鉴定提供参考。【方法】以‘鲁果2号’为鉴定材料,采用表型性状和SSR标记的方法对‘鲁果2号’及其可能的亲本进行亲子分析。【结果】表型性状方法统计的8对坚果性状在各品种间差异很小,不能鉴定出子代亲本;SSR分型检测出疑似亲本中只有‘上宋6号’和‘鲁香’与子代‘鲁果2号’的等位基因遗传符合孟德尔遗传规律,其累积RCP(亲子关系概率)为99.997%,‘slz-40’与已知亲本鉴定结果与实际相符。【结论】‘上宋6号’和‘鲁香’是‘鲁果2号’的亲本,SSR分型技术较好地鉴别出了‘鲁果2号’的亲本,为其它林木和果树品种寻找和鉴别亲本,提供有效手段。

关 键 词:核桃  表型性状  SSR  亲权指数  亲子鉴定

Establishment of Walnut Paternity Test
ZHOU Bei-bei,MA Qing-guo,WANG Hua,WANG Wei,ZHANG Mei-yong,PEI Dong. Establishment of Walnut Paternity Test[J]. Scientia Agricultura Sinica, 2011, 44(20): 4258-4264. DOI: 10.3864/j.issn.0578-1752.2011.20.013
Authors:ZHOU Bei-bei  MA Qing-guo  WANG Hua  WANG Wei  ZHANG Mei-yong  PEI Dong
Affiliation:ZHOU Bei-bei1,MA Qing-guo1,WANG Hua1,WANG Wei1,ZHANG Mei-yong2,PEI Dong1(1Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,2Pomological Institute of Shandong,Taian 271000,Shandong)
Abstract:【Objective】A simple, rapid and efficient walnut paternity method was established to resolve the problems of unknown paternal and confused parents in breeding ,which provided reference for other varieties. 【Method】'Luguo-2' was used as material. Phenotypic traits and SSR markers were conducted to analyse parentage relationship between 'Luguo-2' and its alleged parents.【Result】The phenotypes with statistics of eight nut stable expressional characters failed to get parent-child correlation results. In genotype results, the heredity of 'Shangsong-6', 'Luxiang' and 'Luguo-2' accords with Mendelian genetic regularity, there may be a paternity. PI and RCP wre analyzed by using the results of 10 SSR primer pairs, and the cumulative RCP was 99.997%. In addition, the appraisal result between ‘Slz-40’ and its known parent was consistent with the reality. 【Conclusion】'Shangsong-6' and 'Luxiang' were the parents of 'Luguo-2' with SSR clustering results supporting the conclusion. The parents of 'Luguo-2' were identified by SSR genotypes and thus providing an effective method to search and identify the parents of other trees and fruit varieties.
Keywords:walnut  phenotypes  SSR  PI  paternity test  
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国农业科学》浏览原始摘要信息
点击此处可从《中国农业科学》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号