Development of a Multiplex PCR for Diagnosis of Staphylococcus aureus,Escherichia coli and Bacillus cereus from Cows with Endometritis |
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Authors: | SUN Dong-bo WU Rui HE Xian-jing WANG Shuang LIN Yun-cheng HAN Xu WANG Yue-qiang GUO Ting-ting WU Guo-jun YANG Ke-li |
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Affiliation: | College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, P.R. China |
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Abstract: | Staphylococcus aureus,Escherichia coli and Bacillus cereus are the major agents of cow endometritis in dairy cows.A multiplex PCR (SEB-mPCR) was established based on the conserved genes of S.aureus,E.coli and B.cereus,and the detection limits were 103,102 and 103 CFU mL-1,respectively.SEB-mPCR could not amplify genomic DNA of pathogenic bacteria of other common bovine diseases.A total of 309 vaginal discharge samples from cows with endometritis were tested by SEB-mPCR.Of the samples,23.95% had the three kinds of bacteria detected,17.15% had S.aureu and E.coli,9.39% had E.coli and B.cereus,and 9.71% had S.aureus and B.cereus.The rates of infections with S.aureus,E.coli and B.cereus were 11.35,16.18 and 9.06%,respectively.Therefore,SEB-mPCR has a potential as a diagnosis tool for endometritis in dairy cows. |
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Keywords: | bovine endometritis multiplex PCR S. aureus E. coli B. cereus |
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