中国荷斯坦牛SLC11A1基因多态性与乳腺炎的相关性研究

 【目的】通过研究中国荷斯坦牛SLC11A1基因多态性与乳腺炎性状间的相关性，为奶牛乳腺炎抗性选育提供参考。【方法】采用巢式PCR、降落PCR和DNA测序等技术，研究分析771头牛SLC11A1基因外显子10、内含子9和11的基因多态性，采用SHEsis和PHASE软件进行配对连锁不平衡和单倍型分析。【结果】发现4个不连锁的SNPs，分别为内含子9的6 067（A/G）、6 358（C/T），外显子10的7 155（A/G）和内含子11的7 809（A/T）；其中，6 067（A/G）、6 358（C/T）和7 809（A/T）为新SNPs。不同基因型与乳腺炎相关性分析结果表明，SLC11A1基因的6 358（C/T）和7 155（A/G）对SCS和产奶量有显著影响（P＜0.05），6 358（C/T）的CC基因型和7 155（A/G）的AA基因型是优良基因型；单倍型分析表明，群体中共发现16种单倍型组合，其中CAAA是优良的单倍型组合，其个体的SCS低、产奶量高。【结论】SLC11A1基因CAAA单倍型组合可能是奶牛SCS和产奶量的优良基因型和单倍型组合，可作为分子标记应用于奶牛乳腺炎抗性筛选。

Polymorphisms of SLC11A1 Gene and Their Association with Mastitis in Chinese Holstein

【Objective】The polymorphisms of SLC11A1 gene and their correlation to mastitis in Chinese Holstein were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency of mastitis  resistance. 【Method】CRS-PCR, descend PCR and sequencing were applied to analyze the polymorphisms of four locus in SLC11A1 gene intron 9, exon10 and intron 11 in 771 Chinese Holstein Cattle. The PHASE and SHEsis softwares were used to analyze matching chain disequilibrium and haplotype analysis, respectively. 【Result】 Four SNPs not linking were found, in which 6 067(A/G), 6 358(C/T) and 7 809(A/T) were reported for the first time, and were in intron 9, exon 10 and intron 11, respectively. At locus 6 358 (C/T) and 7 155 (A/G), the cows with genotype CC and AA showed higher milk yield and lower SCS (P＜0.05). Sixteen haplotype combinations were found in 771 Chinese Holstein Cattle, the cows with haplotype combinations CAAA had the higher milk yield and lowest SCS.【Conclusion】Considering the distribution frequencies of haplotype combinations, SLC11A1 gene CAAA haplotype combination is favorable haplotype combinations to the SCS and milk yield and can be chosen as the molecular markers of choosing mastitis resistance selection in dairy cattle.