荧光辅助糖电泳检测葡甘露寡糖条件的分析

 【目的】探索荧光辅助糖电泳（FACE）检测葡甘露寡糖组分的条件。【方法】以魔芋葡甘露寡糖为原料，对比3种荧光衍生剂的电泳分离效果，在此基础上研究分离胶浓度、衍生化反应条件及样品杂质对FACE分离效果的影响，确定FACE分析葡甘露寡糖的最佳条件。【结果】FACE分析葡甘露寡糖最佳条件是：荧光衍生剂为ANTS，分离胶浓度25%，ANTS用量 2 μL，衍生化反应温度37℃，衍生化反应时间16 h。【结论】FACE对葡甘露寡糖组分有很好的分离效果，使用本方法可以确定葡甘露寡糖中至少含有14种组分，为葡甘露寡糖的定性定量分析提供一种有效的检测手段。

Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis

【Objective】 The objective of this study was to explore the conditions of FACE detecting glucomanno- oligosaccharides. 【Method】 Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. 【Result】 The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows: fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 μL, derivation reaction temperature 37℃ and derivation reaction time 16 h.【Conclusion】These findings show that FACE has a high resolution to glucomanno- oligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.