矮牵牛花药培养胚状体诱导和植株再生

[目的]利用矮牵牛的花药培养诱导胚状体和再生植株。[方法]以7个品种矮牵牛的花药为材料,研究其胚状体及其再生植株的诱导方法。[结果]基因型和培养基成分对胚状体的诱导有重要影响;椰乳和活性炭对胚状体的发生及发育有促进作用;向改良MS培养基中添加0.01%活性炭可促进植株再生;最佳生根培养基为1/2MS+IBA 0.2 mg/L。[结论]该研究为矮牵牛的大规模工业化生产奠定了基础。

Embryoid Induction and Plant Regeneration of Petunia hybrida vilm by Its Anther Culture

[Objective] The paper was to induce the embryoid and regenerated plants of Petunia hybrida vilm by its anther culture.[Method] The induction methods of embryoid and regenerated plants were studied by using anther of 7 species of P.hybrida as materials.[Result] Genotype and medium composition had important effects on the induction of embryoid;coconut milk and activated carbon could promote the induction and development of embryoid;the modified MS medium supplemented with 0.01% activated charcoal could promote the regeneration of plants;the best rooting medium was 1/2MS+IBA 0.2 mg/L.[Conclusion] The study laid the foundation for the large-scale industrialization production of P.hybrida.