春石斛兰的茎段繁殖研究

采用春石斛兰的无菌苗作为研究材料,对其茎段繁殖进行系统地研究.采用茎段繁殖可以大大提高上部侧芽诱导率.在春石斛兰侧芽诱导中,较高浓度的无机盐抑制芽的生长;6-BA在0.2～0.8 mg/L的浓度范围内,随着浓度的升高,春石斛兰的侧芽诱导率呈下降趋势;NAA在0.5～1 mg/L的浓度范围内,春石斛兰的侧芽诱导率呈上升趋势.结果表明:春石斛兰茎段诱导侧芽的最适培养基为KC 6-BA 0.2 mg/L NAA 0.5 mg/L或1/2 MS KT 1.5 mg/L NAA 1.0 mg/L.添加香蕉汁等有机附加物有利于侧芽的生长.

Study on Stem Section Reproduction of Dendrobe nobile in Vitro

The Dendrobe nobile in vitro was used as the research material and carried on studies to the stem section reproduction systematically.The stem section reproduction can greatly enhance the inductivity of the lateral bud.Higher consistency of inorganic salt restraints buds' growth in the induction of the lateral bud of Dendrobe nobile.6-BA changes within 0.2~0.8 mg/L consistency range,with the fact that the consistency rises,the inductivity of the lateral bud appears downtrend.NAA consistency range inner in 0.5~1 mg/L,the inductivity of the lateral bud assumes an uptrend.The results indicated that the most suitable culture medium used to induce the lateral bud was KC 6-BA 0.2 mg/L NAA 0.5 mg/L or 1/2 MS KT 1.5 mg/L NAA 1.0 mg/L.Organic additions such as fruit juice of banana were advantageous to lateral bud's growth.